Biology experiments are hard, and so they go wrong, a lot.

For this reason, it is wise to verify that certain steps are correct whenever possible.

And so this is the first thing we did on the second day!

Gel electrophoresis separates molecules by their charge-to-mass ratio. It is one of those ultra common lab procedures!

This allows us to determine how long are the DNA fragments present in our solution.

Since we know that we amplified the 16S regions which we know the rough size of (there might be a bit of variability across species, but not that much), we were expecting to see a big band at that size.

And that is exactly what we saw!

First we had to prepare the gel, put the gel comb, and pipette the samples into wells present in the gel:

To see the DNA, we added ethidium bromide to the samples, which is a substance that that both binds to DNA and is fluorescent.

Because it interacts heavily with DNA, ethidium bromide is a mutagen, and the biology people sure did treat the dedicated electrophoresis bench area with respect! Figure 4. "Gel electrophoresis dedicated bench area to prevent ethidium bromide contamination.".

The UV transilluminator we used to shoot UV light into the gel was the Fisher Scientific UVP LM-26E Benchtop 2UV Transilluminator. The fluorescent substance then emitted a light we can see.

As barely seen at Figure 8. "Fischer Scientific UVP LM-26E Benchtop 2UV Transilluminator illuminated gel." due to bad photo quality due to lack of light, there is one strong green line, which compared to the ladder matches our expected 16S length. What we saw it with the naked eyes was very clear however.

Experiments: speed of light experiments.

This is likely the easiest one to produce as the frequencies are lower, which is why it was discovered first. TODO original setup.

Also because it is transparent to brick and glass, (though not metal) it becomes good for telecommunication.

Some notable subranges:

Once we had the amplified 16S DNA, we were almost ready to start sequencing!

Microwave only found applications into the 1940s and 1950s, much later than radio, because good enough sources were harder to develop.

One notable development was the cavity magnetron in 1940, which was the basis for the original radar systems of World War II.

Apparently, DC current comes in, and microwaves come out.

TODO: sample power efficiently of this conversion and output spectrum of this conversion on some cheap device we can buy today.

Original 1931 experiment by Raman and Bhagavantam: dspace.rri.res.in/bitstream/2289/2123/1/1931%20IJP%20V6%20p353.pdf

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