Working remotely is hard if you don't already highly master the software and enterprise systems used.
Also you don't feel people's love as strongly, and usefulness is built on love, see also Steve Jobs's Pixar office space design philosophy.
But please, give workers a small silent office so that we can concentrate instead of a silly open space, and create an internal social network so people can see what others are doing.
Remote working is much better if the majority of the team also does it, otherwise you will get excluded. Maybe after VR...
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it PCR Updated 2025-06-17 +Created 1970-01-01
More generic PCR information at: Section "Polymerase chain reaction".
Because it is considered the less interesting step, and because it takes quite some time, this step was done by the event organizers between the two event days, so participants did not get to take many photos.
PCR protocols are very standard it seems, all that biologists need to know to reproduce is the time and temperature of each step.
This process used a Marshal Scientific MJ Research PTC-200 Thermal Cycler:
We added PCR primers for regions that surround the 16S DNA. The primers are just bought from a vendor, and we used well known regions are called 27F and 1492R. Here is a paper that analyzes other choices: academic.oup.com/femsle/article/221/2/299/630719 (archive) "Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment" by Yuichi Hongoh, Hiroe Yuzawa, Moriya Ohkuma, Toshiaki Kudo (2003)
One cool thing about the PCR is that we can also add a known barcode at the end of each primer as shown at Code 1. "PCR diagram".
This means that we bought a few different versions of our 27F/1492R primers, each with a different small DNA tag attached directly to them in addition to the matching sequence.
This way, we were able to:
- use a different barcode for samples collected from different locations. This means we
- did PCR separately for each one of them
- for each PCR run, used a different set of primers, each with a different tag
- the primer is still able to attach, and then the tag just gets amplified with the rest of everything!
- sequence them all in one go
- then just from the sequencing output the barcode to determine where each sequence came from!
Input: Bacterial DNA (a little bit)
... --- 27S --- 16S --- 1492R --- ...
|||
|||
vvv
Output: PCR output (a lot of)
Barcode --- 27S --- 16S --- 1492RCode 1.
PCR diagram
. Finally, after purification, we used the Qiagen QIAquick PCR Purification Kit protocol to purify the generated from unwanted PCR byproducts.
As mentioned at the prize was claimed at 8d31992805518fd62daa3bdd2a5c4fd2cd3054c9b3dca1d78055e9528cff6adc (2017-02-23) which spends several inputs with the same unlock script that presents two different constantants that have the same SHA-1:both givingIt was claimed on the same day that Google disclosed the collision: security.googleblog.com/2017/02/announcing-first-sha1-collision.html
printf 255044462d312e330a25e2e3cfd30a0a0a312030206f626a0a3c3c2f57696474682032203020522f4865696768742033203020522f547970652034203020522f537562747970652035203020522f46696c7465722036203020522f436f6c6f7253706163652037203020522f4c656e6774682038203020522f42697473506572436f6d706f6e656e7420383e3e0a73747265616d0affd8fffe00245348412d3120697320646561642121212121852fec092339759c39b1a1c63c4c97e1fffe017f46dc93a6b67e013b029aaa1db2560b45ca67d688c7f84b8c4c791fe02b3df614f86db1690901c56b45c1530afedfb76038e972722fe7ad728f0e4904e046c230570fe9d41398abe12ef5bc942be33542a4802d98b5d70f2a332ec37fac3514e74ddc0f2cc1a874cd0c78305a21566461309789606bd0bf3f98cda8044629a1 | xxd -r -p | sha1sum
printf 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 | xxd -r -p | sha1sumf92d74e3874587aaf443d1db961d4e26dde13e9cBoth of these are PDF prefixes, so they start with the PDF file signature, but are not fully viewable PDFs on their own.
The wiki comments: en.wikipedia.org/w/index.php?title=Ferromagnetism&oldid=965600553#Explanation
The Bohr-van Leeuwen theorem, discovered in the 1910s, showed that classical physics theories are unable to account for any form of magnetism, including ferromagnetism. Magnetism is now regarded as a purely quantum mechanical effect. Ferromagnetism arises due to two effects from quantum mechanics: spin and the Pauli exclusion principle.
Each of the omics studies a subset of molecular biology with a data intensive and broad point of view that tries to understand global function or organisms, trying to understand what every biologically relevant molecule does as part of the hole metabolism.
The main omics are:
Omics might be stamp collecting, but maybe it is a bit more like Trading card game/Magic: The Gathering collecting, in which the cards that you are collecting actually have specific uses and interactions, especially considering that most metabolic pathways are analogous across many species.
Hierarchical diagram of the major omics
. - phaser/hello.html: a minimal hello world adapted from web.archive.org/web/20230323212804/https://phaser.io/tutorials/getting-started-phaser3/part5. Not an actual game strictly speaking though, just shows the phaser logo bouncing around the screen.
- phaser/hello-game.html: an actually hello world game where you have to collect stars and avoid bombs.Based on labs.phaser.io/index.html?dir=games/firstgame/&q=:
- finished version: labs.phaser.io/view.html?src=src/games/firstgame/part10.js
- corresponding tutorial: web.archive.org/web/20230323210501/https://phaser.io/tutorials/making-your-first-phaser-3-game/part10.
And of course, 4chan just takes that to a whole new level, usually closing on the same day, and then getting deleted within a week. Why would anyone contribute non-illegal content to that king of system?!
Ridiculous, so when new information comes out, we just duplicate all the old comments on a new thread again?
Remember, Ciro Santilli is the Necromancer God.
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it Protocols used Updated 2025-06-17 +Created 1970-01-01
- liziyan1117.com/page/:All question PDFs are uploaded to that site. Solutions are scanned from paper notebooks.
These are my own solutions to selected problem sets and past papers of the Oxford MPhys course (Years 1-3) and the MMathPhys course from the years 2014 to 2018
From LinkedIn: - pjcc.physics.ox.ac.uk/resources/notes | www.scribd.com/document/654784089/CP3-Notes-Toby-Adkins# are lectures by Toby Adkins is pointed to from Oxford Physics Joint Consultative Committee. But they are closed, i.e. require you to be in the oxford network, though not necessarily with an Oxford login. As of 2023, he was doing a postdoc: www.physics.ox.ac.uk/our-people/adkins in fusion energy.
The knowledge that light is polarized precedes the knowledge of the existence of the photon, see polarization of light for the classical point of view.
The polarization state and how it can be decomposed into different modes can be well visualized with the Poincaré sphere.
One key idea about photon polarization is that it carries angular momentum. Therefore, when an electron changes orbitals in the Schrödinger equation solution for the hydrogen atom, the angular momentum (as well as energy) change is carried out by the polarization of the photon!
- clear animations showing how two circular polarizations can make a vertical polarization
- a polarizer can be modelled bra operator.
- light polarization experiments are extremely direct evidence of quantum superposition. Individual photons must be on both L and R states at the same time because a V filter passes half of either L or R single photons, but it passes all L + R photons
From the 2020/2021 Oxford physics course handbooks we can determine the following structure:
- Year 1 (CP, "Coure Preliminaries", "Prelims"). Take all of:
- CP1 Classical mechanics, Special relativity
- CP2 Electromagnetism, circuit theory and optics
- CP3 Mathematical methods 1. Complex Numbers and Ordinary Differential Equations. Vectors and Matrices.
- CP4 Mathematical methods 2. Multiple Integrals and Vector Calculus. Normal Modes, Wave Motion and the Wave Equation.
- Year 2 (Part A). Take all of:
- A1 Thermal physics. Kinetic Theory, Heat Transport, Thermodynamics.
- A2 Electromagnetism and optics
- A3 Quantum physics. Quantum Mechanics and Further Quantum Mechanics.
- Short options: at least one of:
- Mathematical Methods
- Probability and Statistics
- S01 Functions of a Complex Variable
- S07 Classical Mechanics
- S10 Medical Imaging and Radiation Therapy
- S13 Teaching and Learning Physics in Schools
- S14 History of Physics
- S20 History of Science
- S21 Philosophy of Science
- S22 Language Options
- S25 Climate Physics
- S27 Philosophy of Space-Time
- S29 Exploring Solar Systems
- S33 Entrepreneurship for Physicists
- Year 3 (Part B). Take all of:
- Michaelmas term
- Hilary term
- B1 Fluids
- B3 Atomic and laser physics
- B5 General relativity
- B7 Classical Mechanics (for MPhysPhil only?)
- B8 Computational Project
- B9 Experimental Project
- Year 4 (MPhys). Select two from:
Trinity term, the third and final term of each year, contains mostly revision from the previous two terms, after which students take their final exams, which basically account for their entire grade. Trinity is therefore a very tense part of the year for the students. After that they have summer holidays, until coming back for the next year of madness.
The official external course landing page: www.ox.ac.uk/admissions/undergraduate/courses/course-listing/physics. 2021 archive: web.archive.org/web/20221208212856/https://www.ox.ac.uk/admissions/undergraduate/courses/course-listing/physics) In those pages we see the rough structure, except that it does not have the course codes "A1" etc., and some courses are missing.
At web.archive.org/web/20221229021312/https://www2.physics.ox.ac.uk/sites/default/files/2011-06-03/course_v3_pdf_80151.pdf page 11 we can see the global course structure giving the two options, 3 year BA or 4 year Oxford physics masters:
Year 1
(Prelims)
|
|
v
Year 2
(Part A)
|
+-----------+
| |
v v
Year 3 BA Year 3 (MPhys)
(Part B) (Part B)
| |
| |
v v
BA Year 4
(Part C)
|
|
v
MPhysPractical courses notes: www-teaching.physics.ox.ac.uk/
Some others with lecture notes:
This is a good book. It is rather short, very direct, which is a good thing. At some points it is slightly too direct, but to a large extent it gets it right.
The main goal of the book is to basically to build the Standard Model Lagrangian from only initial symmetry considerations, notably the Poincaré group + internal symmetries.
The book doesn't really show how to extract numbers from that Lagrangian, but perhaps that can be pardoned, do one thing and do it well.
These often come pre-soldered on devboards, e.g. and allow for easy access to GPIO pins. E.g. they're present on the Raspberry Pi 2.
Why would someone ever sell a devboard without them pre-soldered!
6x1 pin header
. Source. 
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