www.youtube.com/playlist?list=PLVV0r6CmEsFzCodipONmNiROhYCUWyz_U interview with Walter Murch by Web of Stories. He worked on sound design for all The Godfather films, and tells some interesting details about it. He's just super nice in general otherwise. E.g.:
- www.youtube.com/watch?v=w4OK8__gfGo talks about the sound design for the restaurant scene in The Godfather Part II (1974)
"De novo" means "starting from scratch", that is: you type the desired sequence into a computer, and the synthesize it.
The "de novo" part is important, because it distinguishes this from the already well solved problem of duplicating DNA from an existing DNA template, which is what all our cells do daily, and which can already be done very efficiently in vitro with polymerase chain reaction.
Notably, the dream of most of those companies is to have a machine that sits on a lab bench, which synthesises whatever you want.
The initial main applications are likely going to be:but the real pipe dream is building and bootstraping entire artificial chromosomes
- polymerase chain reaction primers (determine which region will be amplified
- creating a custom sequence to be inserted in a plasmid, i.e. artificial gene synthesis
News coverage:
- 2023-03 twitter.com/sethbannon/status/1633848116154880001
- 2020-10-05 www.nature.com/articles/s41587-020-0695-9 "Enzymatic DNA synthesis enters new phase"
Nuclera eDNA enzymatic de novo DNA synthesis explanatory animation (2021)
Source. The video shows nicely how Nuclera's enzymatic DNA synthesis works:- they provide blocked nucleotides of a single type
- add them with the enzyme. They use a werid DNA polymerase called terminal deoxynucleotidyl transferase that adds a base at a time to a single stranded DNA strand rather than copying from a template
- wash everything
- do deblocking reaction
- and then repeat until done
In some systems, e.g. including Metamath, modus ponens alone tends to be enough, everything else can be defined based on it.
CIA 2010 covert communication websites 2013 DNS Census virtual host cleanup heuristic keyword searches Updated 2025-04-24 +Created 1970-01-01
There are two keywords that are killers: "news" and "world" and their translations or closely related words. Everything else is hard. So a good start is:
grep -e news -e noticias -e nouvelles -e world -e global
iran + football:
- iranfootballsource.com: the third hit for this area after the two given by Reuters! Epic.
3 easy hits with "noticias" (news in Portuguese or Spanish"), uncovering two brand new ip ranges:
- 66.45.179.205 noticiasporjanua.com
- 66.237.236.247 comunidaddenoticias.com
- 204.176.38.143 noticiassofisticadas.com
Let's see some French "nouvelles/actualites" for those tumultuous Maghrebis:
- 216.97.231.56 nouvelles-d-aujourdhuis.com
news + global:
- 204.176.39.115 globalprovincesnews.com
- 212.209.74.105 globalbaseballnews.com
- 212.209.79.40: hydradraco.com
OK, I've decided to do a complete Wayback Machine CDX scanning of
news
... Searching for .JAR
or https.*cgi-bin.*\.cgi
are killers, particularly the .jar hits, here's what came out:- 62.22.60.49 telecom-headlines.com
- 62.22.61.206 worldnewsnetworking.com
- 64.16.204.55 holein1news.com
- 66.104.169.184 bcenews.com
- 69.84.156.90 stickshiftnews.com
- 74.116.72.236 techtopnews.com
- 74.254.12.168 non-stop-news.net
- 193.203.49.212 inews-today.com
- 199.85.212.118 just-kidding-news.com
- 207.210.250.132 aeronet-news.com
- 212.4.18.129 sightseeingnews.com
- 212.209.90.84 thenewseditor.com
- 216.105.98.152 modernarabicnews.com
"headline": only 140 matches in 2013-dns-census-a-novirt.csv and 3 hits out of 269 hits. Full inspection without CDX led to no new hits.
He looks like an older and more experienced dude compared to Bezos at the time.
Bibliography:
. www.geekwire.com/2011/meet-shel-kaphan-amazoncom-employee-1/2/ also mentions that unlike California, there's no sales tax in the state of Washington, which is important for selling books.
. www.geekwire.com/2011/meet-shel-kaphan-amazoncom-employee-1/2/ also mentions that unlike California, there's no sales tax in the state of Washington, which is important for selling books.
- a few mentions at: Video "Jeff Bezos presentation at MIT (2002)"
Amazon.com Continues to Grow by NBC 15 (2014)
Source. Features short excerpt of filmed interview with Shel. Unlisted articles are being shown, click here to show only listed articles.