Whenever Ciro's wife starts with "the Asian fetish talk", Ciro reminds her of her own claimed "tall person fetish", and they call it quits.
Asian fetish is much more than sex. It is about the culture, the history, the language. It is the same fetish that makes great mathematicians an Artists do what they do.
When Ciro Santilli was at École Polytechnique, he had to do a presentation for his stupid English courses that all students were forced to take, no matter how good their English was.
The topic was likely something like "pick a country", and Ciro was delighted when he managed to pick "China", after it had gone around a table with many many people before him.
The PowerPoint presentation Ciro made over the weekend was so amazing (and making it was actually fun to make, Ciro actually remembers it a bit!), drawing partly from stuff his wife then girlfriend showed him, that at the end the one of the other students asked him if he had lived in China.
It is a shame Ciro couldn't find the presentation when he wrote this line many many years later. Anything that is not in a BLOB-free monorepo, will disappear, heed my words. But he's certain about two things which it contained:
en.wikipedia.org/wiki/Asian_fetish#History_of_originssubservient, passive, mysterious are understandable. But Hyper-sexual and villainous? OMG have these people never seen real Asians?
en.wikipedia.org/wiki/Asian_fetish#Pornography:
In the United States, women of East and Southeast Asian descent are sometimes stereotyped as subservient, passive, mysterious, villainous in nature, and hyper-sexual.
en.wikipedia.org/wiki/Asian_fetish#Pornography:
The United Kingdom is one big field.
Everything is extremely uniform and fully controlled by humans. Maybe this is partially due to it being an island with extensive flood plains. Loots of white mana floating around there.
Some impressively sounding natural parks look more like cute countryside that is slightly hillier than the surrounding countryside.
This uniformity does however make it quite comfortable for its Hobbit inhabitants.
It also means that whenever slightly out of the ordinary happens, e.g., a bit of slightly heavier rain, everything floods. In some way however, the Brits are very pragmatic, and as long as the flood is not too bad, they just let it be, it might be cheaper.
Decent interactive counties map: help.openstreetmap.org/questions/22603/displaying-uk-ceremonial-counties TODO districts...
Install on Ubuntu 22.04:
python3 -m pip install --user adafruit-ampy How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it Pre-sequencing preparation by 
Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16One cool thing we did in this procedure was to use magnetic separation with magnetic beads to further concentrate the DNA: Figure 1. "GE MagRack 6 pipetting.".
The beads are coated to stick to the DNA, which allows us to easily extract the DNA from the rest of the solution. This is cool, but bio people are borderline obsessed by those beads! Go figure!
Then we prepared the DNA for sequencing with the Oxford Nanopore specific part: Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit.
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it PCR verification with gel electrophoresis by Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16For this reason, it is wise to verify that certain steps are correct whenever possible.
Gel electrophoresis separates molecules by their charge-to-mass ratio. It is one of those ultra common lab procedures!
Since we know that we amplified the 16S regions which we know the rough size of (there might be a bit of variability across species, but not that much), we were expecting to see a big band at that size.
And that is exactly what we saw!
First we had to prepare the gel, put the gel comb, and pipette the samples into wells present in the gel:
To see the DNA, we added ethidium bromide to the samples, which is a substance that that both binds to DNA and is fluorescent.
Because it interacts heavily with DNA, ethidium bromide is a mutagen, and the biology people sure did treat the dedicated electrophoresis bench area with respect! Figure 4. "Gel electrophoresis dedicated bench area to prevent ethidium bromide contamination.".
Gel electrophoresis dedicated bench area to prevent ethidium bromide contamination.
Source. 
Gel electrophoresis dedicated waste bin for centrifuge tubes and pipette tips contaminated with ethidium bromide.
Source. The UV transilluminator we used to shoot UV light into the gel was the Fisher Scientific UVP LM-26E Benchtop 2UV Transilluminator. The fluorescent substance then emitted a light we can see.
As barely seen at Figure 8. "Fischer Scientific UVP LM-26E Benchtop 2UV Transilluminator illuminated gel." due to bad photo quality due to lack of light, there is one strong green line, which compared to the ladder matches our expected 16S length. What we saw it with the naked eyes was very clear however.
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it Post filtration purification by Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16After filtration, all DNA should present in the filter, so we cut the paper up with scissors and put the pieces into an Eppendorf: Video 1. "Cutting vacuum pump filter and placing it in Eppendorf".
Cutting vacuum pump filter and placing it in Eppendorf
. Source. Now that we had the DNA in Eppendorfs, we were ready to continue the purification in a simpler and more standardized lab pipeline fashion.
First we added some small specialized beads and chemicals to the water and shook them Eppendorfs hard in a Scientific Industries Inc. Vortex-Genie 2 machine to break the cell and free the DNA.
Once that was done, we added several reagents which split the solution into two phases: one containing the DNA and the other not. We would then pipette the phase with the DNA out to the next Eppendorf, and continue the process.
In one step for example, the DNA was present as a white precipitate at the bottom of the tube, and we threw away the supernatant liquid: Figure 1. "White precipitate formed with Qiagen DNeasy PowerWater Kit".
At various stages, centrifuging was also necessary. Much like the previous vacuum pump step, this adds extra gravity to speed up the separation of phases with different molecular masses.
Then, when we had finally finished all the purification steps, we measured the quantity of DNA with a Biochrom SimpliNano spectrophotometer to check that the purification went well:
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