How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it External links to this page by 
Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16- 2021-03-25: Oxford Nanopore Technologies retweeted this article, that's awesome!
- 2021: hackaday.com/author/wd5gnr1/ "SEQUENCING DNA FOR METAGENOMICS" by Al Williams (2021). This came after Ciro Santilli self promoted at: stackoverflow.blog/2021/02/03/sequencing-your-dna-with-a-usb-dongle-and-open-source-code/#comment-1411921
Oxford Master Course in Mathematical and Theoretical Physics by Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16Microwave only found applications into the 1940s and 1950s, much later than radio, because good enough sources were harder to develop.
One notable development was the cavity magnetron in 1940, which was the basis for the original radar systems of World War II.
As of 2021, last commit from 2017.
Running:failed on Ubuntu 20.10 Node.js v14.15.3 with:Likely similar bullshit from: stackoverflow.com/questions/50111688/node-sqlite-node-gyp-build-error-no-member-named-forceset-in-v8object because the Node.js version is too new.
git clone https://github.com/Codaisseur/feathersjs-react-redux-ssr
cd feathersjs-react-redux-ssr
npm install../src/create_string.cpp:17:37: error: no matching function for call to ‘v8::String::Utf8Value::Utf8Value(v8::Local<v8::Value>&)’
17 | v8::String::Utf8Value string(value);
| ^If I try
nvm install v10I Google error messages until reaching:and the next problem is: stackoverflow.com/questions/48513573/gulp-error-gulp-hastask-is-not-a-function
diff --git a/gulpfile.js b/gulpfile.js
index b931e06..24d2cc8 100644
--- a/gulpfile.js
+++ b/gulpfile.js
@@ -14,34 +14,34 @@ gulp.task('css', function() {
.pipe(gulp.dest('./dist'))
})
-gulp.task('css:watch', ['css'], function() {
+gulp.task('css:watch', gulp.series('css', function() {
gulp.watch('app/styles/**/*.sass', ['css'])
-})
+}))
gulp.task('moveAssets', function() {
return gulp.src('./app/assets/**/*')
.pipe(gulp.dest('./dist/assets'))
})
-gulp.task('build:revAssets', ['css', 'moveAssets'], function() {
+gulp.task('build:revAssets', gulp.series('css', 'moveAssets', function() {
var rev = new $.revAll()
return gulp.src('./dist/**/*')
.pipe(rev.revision())
.pipe(gulp.dest('./dist/public'))
.pipe(rev.manifestFile())
.pipe(gulp.dest('./dist'))
-})
+}))
gulp.task('build:cpServer', function() {
return gulp.src('./app/**/*.{js,ejs}')
.pipe(gulp.dest('./dist/server-build'))
})
-gulp.task('build:revServer', ['build:cpServer'], function() {
+gulp.task('build:revServer', gulp.series('build:cpServer', function() {
var manifest = gulp.src('./dist/rev-manifest.json')
return gulp.src('./dist/server-build/{components,containers}/**/*')
.pipe($.revReplace({ manifest: manifest }))
.pipe(gulp.dest('./dist/server-build'))
-})
+}))
gulp.task('build', function() {
runSequence('build:revAssets', 'build:revServer')
diff --git a/package.json b/package.json
index bcb29c3..86bd593 100644
--- a/package.json
+++ b/package.json
@@ -67,7 +67,7 @@
"redux-thunk": "^0.1.0",
"request": "^2.79.0",
"rewire": "^2.3.4",
- "run-sequence": "^1.2.2",
+ "run-sequence": "^2.2.1",
"serve-favicon": "^2.3.2",
"socket.io-client": "^1.7.2",
"superagent": "^1.4.0",
@@ -86,16 +86,16 @@
"concurrently": "^2.0.0",
"cross-env": "^1.0.7",
"enzyme": "^2.3.0",
- "gulp": "^3.9.0",
+ "gulp": "^4.0.2",
"gulp-autoprefixer": "^3.1.0",
"gulp-load-plugins": "^1.2.0",
"gulp-rev": "^6.0.1",
- "gulp-sass": "^2.1.1",
+ "gulp-sass": "4.1.0",
"gulp-sourcemaps": "^1.6.0",
"jsdom": "^7.0.1",
"mocha": "^2.4.5",
"nock": "^2.17.0",
- "node-sass": "^3.4.2",
+ "node-sass": "^5.0.0",
"nodemon": "^1.6.0",
"react-addons-test-utils": "^15.3.2",
"react-transform-catch-errors": "^1.0.0",The first gene in the E. Coli K-12 MG1655 genome. Remember however that bacterial chromosome is circular, so being the first doesn't mean much, how the choice was made: Section "E. Coli genome starting point".
Part of E. Coli K-12 MG1655 operon thrLABC.
At only 65 bp, this gene is quite small and boring. For a more interesting gene, have a look at the next gene, e. Coli K-12 MG1655 gene thrA.
Does something to do with threonine.
This is the first in the sequence thrL, thrA, thrB, thrC. This type of naming convention is quite common on related adjacent proteins, all of which must be getting transcribed into a single RNA by the same promoter. As mentioned in the analysis of the KEGG entry for e. Coli K-12 MG1655 gene thrA, those A, B and C are actually directly functionally linked in a direct metabolic pathway.
We can see that thrL, A, B, and C are in the same transcription unit by browsing the list of promoter at: biocyc.org/group?id=:ALL-PROMOTERS&orgid=ECOLI. By finding the first one by position we reach; biocyc.org/ECOLI/NEW-IMAGE?object=TU0-42486.
Transcription factor for E. Coli K-12 MG1655 operon thrLABC as shown at biocyc.org/ECOLI/NEW-IMAGE?object=TU0-42486.
Note that this is very close to the "end" of the genome.
Research group of the Department of physics of the University of Oxford by Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16 Superconducting qubits are bad because it is harder to ensure that they are all the same by Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16This is unlike atomic systems like trapped ion quantum computers, where each atom is necessarily exactly the same as the other.
Unlisted articles are being shown, click here to show only listed articles.