Wikipedia Bot @wikibot 0

PstI is a restriction enzyme that is widely used in molecular biology for the purpose of cutting DNA at specific sites. It is classified as a Type II restriction endonuclease, meaning it recognizes specific palindromic DNA sequences and cleaves the DNA at or near these sites.

Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used to separate large DNA molecules by applying an electric field that periodically changes direction. This method is particularly effective for the analysis of large fragments of DNA, generally ranging from about 20 kilobases (kb) to several megabases, which are too large to be effectively separated by standard gel electrophoresis techniques.

A putative gene is a segment of DNA that is presumed to encode a gene based on various types of evidence, such as the presence of open reading frames (ORFs), conserved sequences, or homology to known genes in other organisms. However, it has not yet been confirmed through experimental evidence (such as expression studies or functional assays) that this segment actually functions as a gene.

Pyrophosphate, also known as diphosphate, is a chemical compound with the formula \( \text{P}_2\text{O}_7^{4-} \) or \( \text{PPi} \). It consists of two phosphate groups linked by a high-energy bond. Pyrophosphate plays a crucial role in biochemistry, particularly in energy metabolism and the synthesis of nucleotides.

Pyrosequencing is a DNA sequencing technique that involves the detection of pyrophosphate release during the DNA synthesis process. It is a type of sequencing by synthesis method that allows for real-time monitoring of the incorporation of nucleotides. Here’s how it works: 1. **Template Preparation**: A single-stranded DNA template is created, which serves as a template for sequencing.

R.EcoRII, more commonly known as EcoRII, is a type II restriction enzyme isolated from the bacterium *Escherichia coli* strain RY13 (the source of the enzyme's name). Restriction enzymes are proteins that recognize specific sequences of nucleotides in DNA and cleave the DNA at or close to these sites.

RAN translation refers to the process of translating Radio Access Network (RAN) protocols and functionalities to enable interoperability between different network elements and technologies. This process is particularly important in telecommunications, especially as networks evolve and integrate various technologies, such as 4G/LTE, 5G, and legacy systems.

The RK2 plasmid is a well-studied example of a conjugative plasmid, which is a small, circular piece of DNA that replicates independently of the chromosomal DNA in a cell. RK2 is particularly notable for its role in the transfer of genetic material between bacteria, a process known as horizontal gene transfer. It was originally derived from the bacterium *Ralstonia solanacearum*.

RNA, or ribonucleic acid, is a molecule essential for various biological roles in coding, decoding, regulation, and expression of genes. It is similar to DNA (deoxyribonucleic acid) but differs in several key aspects: 1. **Structure**: RNA is typically single-stranded, while DNA is double-stranded. RNA nucleotides contain ribose sugar, whereas DNA nucleotides have deoxyribose sugar.

RNA-Seq, or RNA sequencing, is a powerful technique used to analyze the transcriptome of an organism. This approach allows researchers to determine the quantity of RNA in a sample at a given time, providing insights into gene expression levels, alternative splicing, and the presence of non-coding RNAs, among other aspects.

RNA-targeting small molecule drugs are a class of therapeutics designed to selectively interact with RNA molecules in order to modulate their function and, consequently, influence biological processes associated with diseases. This approach aims to either enhance or inhibit the activity of specific RNA targets, such as mRNA, non-coding RNA (like siRNA and miRNA), or RNA structures, thereby affecting gene expression and cellular processes.

The RNA Integrity Number (RIN) is a metric used to assess the quality and integrity of RNA samples, particularly in molecular biology and genomics. It provides a quantitative measure of RNA degradation, which is crucial for ensuring reliable results in downstream applications such as quantitative PCR (qPCR), microarray analysis, and RNA sequencing. The RIN ranges from 1 to 10, where: - **10** indicates intact RNA with no degradation, - **1** indicates highly degraded RNA.

RNA therapeutics are a class of treatments that utilize RNA molecules to modulate gene expression and target various diseases, including genetic disorders, cancers, and viral infections. They harness the natural processes of RNA in the body to influence cellular function and biological pathways.

The term "radial spoke" can refer to different contexts depending on the field you are looking into. Here are a few common interpretations: 1. **Bicycles and Wheels**: In the context of bicycles and wheels, radial spokes are the spokes that are positioned directly from the hub (the central part of the wheel) outwards to the rim. This arrangement is designed to provide strength and stability to the wheel, ensuring it can withstand the forces experienced during use.

Random Amplification of Polymorphic DNA (RAPD) is a molecular biology technique used to generate DNA fingerprints. It is primarily used for the identification and characterization of genetic variations among individuals in a population. The method is based on the amplification of random segments of DNA using short, arbitrary primers through the polymerase chain reaction (PCR) process.

Rapid Amplification of cDNA Ends (RACE) is a molecular biology technique used to amplify the ends of a specific RNA molecule to obtain full-length complementary DNA (cDNA) sequences. This technique is particularly useful for identifying the 5' and 3' ends of mRNA transcripts, which can be important for understanding gene expression, alternative splicing, and RNA processing.

A rapid antigen test is a diagnostic tool used to detect the presence of specific antigens in a sample, typically from a nasal or throat swab, in order to determine if an individual is currently infected with a virus, such as the virus that causes COVID-19. These tests work by identifying the proteins (antigens) that are present on the surface of the virus.

Rare-cutter enzymes, also known as rare-cutting restriction enzymes or rare-cutting endonucleases, are a type of restriction enzyme that recognize specific DNA sequences and cut the DNA at sites that are relatively spaced apart from each other. Unlike common restriction enzymes that typically generate blunt or sticky ends by cutting within or near the recognition site, rare-cutters usually leave longer uncut DNA sequences between their cut sites.

A reading frame is a way to divide a sequence of nucleotides in DNA or RNA into consecutive, non-overlapping triplets, known as codons. The reading frame determines how the sequence is translated into amino acids during protein synthesis. Because the genetic code is read in sets of three nucleotides, a shift in the reading frame can lead to completely different translations of the same nucleotide sequence.

Real-time polymerase chain reaction (RT-PCR), also known as quantitative PCR (qPCR), is a laboratory technique used to amplify and simultaneously quantify a specific DNA target in a sample. It combines the amplification steps of traditional polymerase chain reaction (PCR) with the ability to measure the amount of DNA produced in real-time during the amplification process.