Ellipsoid rotational diffusion microscopy experiment

In 2006, Yilong Han and colleagues dispersed plastic ellipsoids in water inside a thin glass cell, using a very dilute suspension to limit particle interactions. A camera on an optical microscope recorded 30 frames per second. Image analysis extracted centre positions and long-axis angles, with reported angular resolution about $1^{\circ }$. Their shape supplied the observable axis. Reflected interference colours calibrated cell thickness; nearly constant particle interference colour checked vertical wandering. One tracked particle had dimensions $2.4\times 0.3\times 0.3\mu \mathrm{m}$ in an $846\mathrm{nm}$ gap. Angular displacement variance grew linearly with lag; displacement resolved along the particle's axes and in the laboratory showed how turning washes out translational anisotropy. These are observations of confined particles: walls alter drag, and slight out-of-plane tumbling affected positional resolution. They do not establish the bulk sphere formula. The paper's Figure 1A–B shows simulations; Figure 1F shows a measured trajectory.^[ref]

For interpreting such images, the director distinction is essential: identical ends give an angle modulo $\pi$. A continuously unwrapped trace can follow small successive turns, but it does not make the ends physically distinguishable. The invariant planar axis autocorrelation is $⟨\cos (2\Delta \varphi )⟩$, and its decay determines $D_r$ through $e^{-4D_{r}t}$. Separately measuring position and rigid body orientation is what makes the connection between translational anisotropy and loss of angular memory experimentally accessible.