Empty magnification

Empty magnification enlarges an image without revealing additional specimen detail. Adequate angular magnification can make existing detail visible to the eye, but it cannot undo the objective's diffraction blur. For equally bright, mutually incoherent optical point sources and an ideal circular optical pupil, a commonly used lateral Rayleigh criterion in object space iswhere $\lambda$ is the vacuum wavelength, $n$ is the specimen-medium refractive index, and $\alpha$ is the admitted half-angle. For this model, $\lambda =550\mathrm{nm}$ and $\mathrm{NA}=0.25$ give $d_{\mathrm{R}}\simeq 1.342\mu \mathrm{m}$; changing only numerical aperture to $0.50$ gives $0.671\mu \mathrm{m}$. Changing only the eyepiece leaves this object-space scale unchanged. Illumination, optical coherence, optical aberrations and specimen visibility matter in real instruments; this criterion is not a universal threshold.^[ref]

The geometric reason is especially simple. If a fixed recorded image is enlarged by a factor $q>0$, a feature separation $s$ and a blur width $b$ become $qs$ and $qb$. Their ratio remainsThis is the lower-panel construction in the diagram. A change to the microscope objective can change numerical aperture and hence the specimen detail captured; a display zoom changes how large that captured detail looks. Microscope scale calibration, diffraction-limited resolution and angular magnification are three complementary properties to report.