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A knot invariant assigns the same value to isotopic knots. Examples include the knot group, Alexander polynomial of a knot, Seifert genus, knot signature and Jones polynomial. Different knot invariants can retain different information: isomorphic knot groups do not alone imply isotopic knots.

Knot theory studies closed curves in three-dimensional Euclidean space that cannot intersect themselves, and asks which can be deformed into one another without cutting or passing one strand through another. Its basic object is a knot: a smooth embedding of a circle, considered up to ambient isotopy. Several disjoint knots form a link. More generally, knot theory studies such embeddings in other manifolds and in higher dimensions.

The central surprise is that a flexible circle can carry permanent information about how it sits in space. A trefoil knot and an unknot each consist of just one circle, but no ambient isotopy turns one into the other. A knot diagram translates this spatial question into a finite drawing; Reidemeister moves describe the changes of drawing that preserve the knot. Knot invariants translate it into computations: unequal Alexander polynomials of a knot, Jones polynomials, or knot groups certify different knots. Equal values of a particular knot invariant generally leave the question open; even an isomorphism of knot groups alone is not a complete classification.

An early foundation for working with knot diagrams is Kurt Reidemeister's 1927 paper Elementare Begründung der Knotentheorie, published in volume 5 of Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg, pages 24–32.^[ref] Marc Lackenby's authored notes Elementary knot theory, Section 2.4, explain the Reidemeister move theorem and its role in searching for a certificate that two knot diagrams represent the same knot.^[ref]

Orient $S^3$ as the boundary of $B^4$. Push the interior of an oriented Seifert surface $F$ slightly into $B^4$, and let $X_F$ be the double branched covering of $B^4$ over this pushed-in surface. Its boundary is the two-fold branched cover of a knot. Define the knot signature byThe signature of a possibly degenerate intersection form means the number of positive eigenvalues minus the number of negative eigenvalues; its radical contributes zero.

Here is why the definition is independent of $F$. Given two choices $F_0,F_1$, glue their branched covers along the common boundary to obtainThis is the double branched covering of $S^4$ over the closed oriented surface obtained by gluing the pushed-in $F_0$ to $-F_1$. Every closed oriented surface in $S^4$ admits a Seifert hypersurface: its complement has an integral meridional class, represent it by a map to the circle, and take a regular value. Near the surface choose the angular map in its trivial normal disk bundle. The closure of a suitable regular fiber is a compact oriented three-manifold bounding the surface. Its trivial normal framing can be chosen to match this construction.

Push the interior of this Seifert hypersurface into $B^5$. The double branched covering of $B^5$ over the resulting properly embedded three-manifold has boundary $X$. The existence of the cover follows from the meridian homomorphism to $\mathbb{Z}/2$; smoothness near the branch set is the local map $(z,u)\mapsto (z^2,u)$. By the hypothesis about boundaries of five-manifolds, $\mathrm{sign}X=0$. Novikov additivity for gluing along an entire closed three-dimensional boundary givesThus the knot signature is independent of the Seifert surface. An isotopy of the knot carries the construction to an equivalent one, so it is also a knot invariant.

To calculate its intersection form, express $F$ as a disk with $2g$ bands. The double branched covering of $B^4$ over the pushed-in disk is again $B^4$. Each band lifts to a two-handle. The capped lifted cores give a basis of $H_2(X_F;\mathbb{Z})$ corresponding to the band-core basis of $H_1(F;\mathbb{Z})$. The framing of the $i$th lifted attaching circle is $2\mathrm{lk}(a_i^{+},a_i)=2V_{ii}$. For distinct bands, the two sheets contribute the two push-off linkings $V_{ij}$ and $V_{ji}$, so the mutual linking number is $V_{ij}+V_{ji}$. HenceA different integral basis changes this matrix by matrix congruence and leaves its signature unchanged.

In Figure 7 the upper knot is a connected sum of knots $T\#T$, while the lower one is $T\#\overline{T}$, where $T$ is the positive trefoil knot. Both knot groups have the same presentationTo see this, the Seifert-van Kampen theorem expresses the knot group of a connected sum of knots as the amalgamated free product of the two summand knot groups, amalgamating their meridians of a knot. A trefoil knot has presentation $⟨a,b\mid aba=bab⟩$ with $a$ a meridian. Reflection gives the same abstract group for the mirror. If it reverses the chosen meridian, send both generators to their inverses; the inverse braid relation is again the same relation. Thus the meridian amalgamation yields the displayed group presentation in both cases.

A band basis for the positive trefoil knot giveswhose symmetrization is negative definite. Therefore $\sigma (T)=-2$ and $\sigma (\overline{T})=2$. Boundary-connected-summing Seifert surfaces makes the Seifert matrix block diagonal, so the knot signature is additive under connected sum of knots. ConsequentlyThe upper knot and lower knot are therefore not isotopic, even though their knot groups are isomorphic. With the opposite global knot signature convention the first value is $+4$, and the distinction is unchanged.

The trefoil is the torus knot $T_{2,3}$. It is the simplest nontrivial knot by crossing number of a knot: $c(T_{2,3})=3$. A standard three-crossing alternating knot diagram is reduced, so the Tait crossing-number theorem establishes minimality. The four-crossing knot diagram shown below also represents the trefoil knot, illustrating that a particular projection need not achieve that minimum. Its Seifert genus is one.

Here is a small algebraic certificate that a trefoil knot cannot be deformed into an unknot. One Seifert matrix for a trefoil knot isTaking the determinant gives the Alexander polynomial of a knotwhere $\doteq$ allows multiplication by a Laurent unit $\pm t^n$ in the Laurent polynomial ring. No such multiplication turns this three-term Alexander polynomial of a knot into the unknot's value $1$. Since the Alexander polynomial of a knot is a knot invariant, this computation rules out every possible ambient isotopy between the two knots; it does not merely rule out the deformations we happened to try.

The knot group supplies another certificate. The presentation $⟨a,b\mid a^2=b^3⟩$ maps onto the symmetric group $S_3$ by $a\mapsto (12)$ and $b\mapsto (123)$, since both sides of the relation map to the identity. These images generate a non-abelian group, so the knot group cannot be the unknot's infinite cyclic group. However, a trefoil knot and its mirror of a link have isomorphic knot groups: the abstract knot group alone does not distinguish all knots.