An Escherichia coli transcription unit encoding lactose-use proteins. Expression integrates LacI-dependent repression, allolactose induction, catabolite activator protein activation and inducer exclusion.
IPTG is a nonmetabolizable lactose analogue used as an inducer of the lac operon. Binding changes LacI's affinity for the lac operator; it supplies a way to change repressor availability without assuming that cytoplasmic transport is instantaneous.
A DNA recognition sequence bound by LacI. Repeated copies are used in fluorescent operator-repressor chromosome labelling.

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  1. Regulation of gene expression
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