Live-cell imaging follows living biological cells over time. Labels, illumination and immobilization must be checked for effects on the process being measured; an apparently sharp image is not sufficient evidence that the cell remains physiologically unperturbed.
Imaging of biological events in a living organism. Repeated cell tracking can establish event ordering but requires controls for phototoxicity, imaging access and sampling.

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  1. Fluorescence microscopy
  2. Fluorescence microscope
  3. Fluorescence
  4. Physical optics
  5. Optics
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