Past exam of the chemistry course of the year 3 of the natural sciences course of the University of Cambridge / 2021 / Paper 2A / Section B / 22 / a / i

For the absorption spectrum, pass a scanned monochromatic beam through the solution and a matched solvent reference. Record the incident and transmitted intensities and calculate the absorbance $A(\lambda )={\log }_{10}[I_0(\lambda )/I(\lambda )]$, using an appropriate blank and concentration to avoid saturation.

For the fluorescence emission spectrum, excite at a fixed wavelength within an absorption band, collect emission away from the incident beam, commonly at $90^{\circ }$, and scan an emission monochromator. Correct the detected signal for wavelength-dependent detector sensitivity, subtract solvent/background light and exclude scattered excitation light. The two plotted vertical scales have been independently normalized; their equal peak heights do not imply equal absorption and emission rates or unit fluorescence quantum yield.