Without quencher, . Taking the ratio gives the Stern-Volmer equationThus the plot has unit intercept and slope . A triplet formation yield less than one would cancel from this ratio if it remained independent of quencher concentration. The result assumes dynamic triplet quenching and no additional quencher-induced absorption or singlet loss.[ref]
Stern-Volmer kinetic relationships in the IUPAC Gold Book. Definition and dynamic-quenching relation between quantum-yield ratios, quencher concentration and unquenched lifetime
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