Rate-zonal centrifugation resolves an initially narrow sample zone by differences in sedimentation coefficient during a finite interval. Its bands remain transient: extending the interval can carry material into a centrifugation pellet. Thus a density gradient need not imply an equilibrium buoyant density measurement.[ref]
https://www.beckman.com/resources/technologies/centrifugation/density-gradient-centrifugation/. Types of Density Gradients, Rate Zonal Centrifugation and Table 1: migration rates before equilibrium or complete sedimentation
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