Sinusoidal-stage optical trap calibration ($\beta =u_0/[A_s\sqrt{(2\pi f_c{)}^2+{\Omega }^2}]$)

For an overdamped harmonic optical trap, impose a known sinusoidal local fluid velocity of amplitude $u_0$ and angular frequency $\Omega$. The coherent bead response has amplitude $A_x=u_0/\sqrt{(k/\zeta {)}^2+{\Omega }^2}$. Measuring the thermal corner frequency $f_c=k/(2\pi \zeta )$ and detector-voltage amplitude $A_s=A_x/\beta$ therefore determines the position sensitivity in the displayed formula without a drag coefficient as input. Use a thermal record with the drive line removed to obtain stiffness from the equipartition theorem. Fluid-following, detector response and hydrodynamic corrections must remain valid. Combined thermal and stage measurements have experimentally calibrated an optical trap without drag as an input.^[ref]