DNA detection by Ciro Santilli 40 Updated 2025-07-16
DNA detection means determining if a specific DNA sequence is present in a sample.
This can be used to detect if a given species of microorganism is present in a sample, and is therefore a widely used diagnostics technique to see if someone is infected with a virus.
You could of course do full DNA Sequencing to see everything that is there, but since it is as a more generic procedure, sequencing is more expensive and slow.
The alternative is to use a DNA amplification technique.
DNA amplification by Ciro Santilli 40 Updated 2025-07-16
DNA amplification is one of the key DNA technologies:
An implicit surface is a type of surface defined by an equation that expresses the relationship between the coordinates of points in a space. Specifically, an implicit surface in three-dimensional space can be described using a function \( F(x, y, z) \).
AMPL (A Mathematical Programming Language) is a high-level programming language designed for modeling and solving mathematical optimization problems. It provides a user-friendly syntax that is similar to mathematical notation, making it easier for users to formulate mathematical models for problems in various fields, including operations research, engineering, finance, and logistics. Key features of AMPL include: 1. **Modeling Capability**: AMPL allows users to define variables, constraints, and objective functions in a clear and concise manner.
Also known as: Quantitative PCR (qPCR).
Like PCR, but the amplification machine measures the concentration of DNA at each step.
This describes one possible concentration detection method with fluorescent molecules that only become fluorescent when the DNA is double stranded (SYBR Green)
Video 1.
Polymerase Chain Reaction (PCR) - Quantitative PCR (qPCR) by Applied Biological Materials (2016)
Source.
This allows you to predict the exact initial concentration by extrapolating the exponential curve backwards.
TODO: vs non-real-time PCR. Why can't you just divide by 2 for every heating step to reach back the original concentration? Likely the reaction reach saturation at an unknown step.
TODO: vs non-real-time PCR in medical diagnostics: do you really need to know concentration for diagnostics? Isn't it enough to know if the virus is present or not?
The term "Intermediate Data Format" (IDF) can refer to different contexts depending on the field, but it generally denotes a format used for data that acts as a bridge between two systems or processes. It allows data to be transformed, interpreted, or processed without being directly tied to specific input or output formats.
An isophote is a line or contour on a diagram or image that connects points of equal brightness or intensity. In the context of astronomical imaging and other fields such as photography and remote sensing, isophotes are used to represent the distribution of light intensity across a surface, like that of a galaxy, star, or other celestial objects. In astronomy, isophotes can help in studying the spatial distribution of stars within galaxies, the morphology of galaxies, and the characteristics of nebulae.
OpenStructures is an initiative and platform that promotes modular design and open-source principles for creating physical objects and systems. The concept encourages the development of modular products that can be easily customized, repaired, and maintained by users. At the core of OpenStructures is the idea that products should be designed in a way that allows for the interchangeability of parts, fostering a community-driven approach where individuals can share designs, adapt existing structures, and innovate collaboratively.
The Russian Geometric Kernel (RGK) is a mathematical framework primarily used in the field of computational geometry. It is designed for geometric computations and incorporates several mathematical constructs that facilitate manipulations of geometric entities such as points, lines, polygons, and polyhedra. One of the key features of the RGK is its ability to handle geometric operations and queries with considerations for numerical stability and robustness. This includes managing issues related to precision, which can be critical in computational geometry applications.
Surface triangulation is a computational method used in computer graphics, computational geometry, and mesh processing to divide a surface into a set of triangles. This is important for various applications, including 3D modeling, rendering, simulations, finite element analysis, and more. Here are some key points about surface triangulation: 1. **Purpose**: The main purpose of surface triangulation is to simplify the representation of complex surfaces.

Pinned article: Introduction to the OurBigBook Project

Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
We have two killer features:
  1. topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculus
    Articles of different users are sorted by upvote within each article page. This feature is a bit like:
    • a Wikipedia where each user can have their own version of each article
    • a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
    This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
    Figure 1.
    Screenshot of the "Derivative" topic page
    . View it live at: ourbigbook.com/go/topic/derivative
  2. local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:
    This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
    Figure 2.
    You can publish local OurBigBook lightweight markup files to either https://OurBigBook.com or as a static website
    .
    Figure 3.
    Visual Studio Code extension installation
    .
    Figure 4.
    Visual Studio Code extension tree navigation
    .
    Figure 5.
    Web editor
    . You can also edit articles on the Web editor without installing anything locally.
    Video 3.
    Edit locally and publish demo
    . Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension.
    Video 4.
    OurBigBook Visual Studio Code extension editing and navigation demo
    . Source.
  3. https://raw.githubusercontent.com/ourbigbook/ourbigbook-media/master/feature/x/hilbert-space-arrow.png
  4. Infinitely deep tables of contents:
    Figure 6.
    Dynamic article tree with infinitely deep table of contents
    .
    Descendant pages can also show up as toplevel e.g.: ourbigbook.com/cirosantilli/chordate-subclade
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact