Ciro Santilli @cirosantilli 37

It is rare to find a project with such a ridiculously high importance over funding ratio.

E.g., as of 2020, their help login help.openstreetmap.org/ shows MyOpenID as an option, which was discontinued in 2014, and not Google OAuth.

They do still seem to have a bit more activity than gis.stackexchange.com/questions/tagged/openstreetmap on Stack Exchange.

Complaints:

All of this is a shame, because they do have some incredible data that you cannot find easily on other maps because people just edited it up.

Not really dedicated to open source course material, nor to free courses...

The "Open" in its name only made sense in the 60's, when it was founded, nowadays, there isn't much about this institution that is very different compared to traditional Oxbridge. "Cheap more online university" would be a more adequate name for it.

A system that would truly live up to the name "Open" in the year 2020 is the one described at the ideal university by Ciro Santilli.

Wikipedia even says that the initial focus was on broadcasting learning material on television and radio, so what happened to that now that we have an even more powerful on-demand tool called Internet!

They even created their own MOOC website, FutureLearn. But www.freecodecamp.org/news/massive-open-online-courses-started-out-completely-free-but-where-are-they-now-1dd1020f59/ mentions:OMG. God why.

A few open sources at: www.open.edu/openlearn/free-courses. The 5-hour course on particle physics says it all. Stated as of 2023 at www.open.ac.uk/about/open-educational-resources/openlearn/free-learning:

www.youtube.com/watch?v=Pj0rbafFBak What's an Open University Degree Like? by Luke Cutforth (2021) mentions that it is more autodidactic/online, and it encourages part time learning.

youtu.be/rsWwffX-u0A?t=99 Open University - How does it work? by Matt Greg Vlogs (2017) shows that they do have their own custom institutional material. And it is not open???? Please. youtu.be/rsWwffX-u0A?t=222 mentions that there is no entry exam, and you can change your courses at any time, that is good at least.

Israel apparently also created their own version in the 70's inspired by the British one: Open University of Israel. Same story it seems.

Consider the E. Coli K-12 MG1655 operon thrLABC.

That single operon can produce two different mRNA transcription units:

The reason for this appears to be that there is a rho-independent termination region after thrL. But then under certain conditions, that must get innactivated, and then the thrLABC is produced instead.

Start with consulting for universities to get some cash flowing.

Help teachers create perfect courses.

At the same time, develop the website, and use the generated content to bootstrap it.

Choose a domain of knowledge, generate perfect courses for it, and find all teachers of the domain in the world who are teaching that and help them out.

Then expand out to other domains.

TODO: which domain of knowledge should we go for? The more precise the better.

Crush the current grossly inefficient educational system, replace today's students + teachers + researchers with unified "online content creators/consumers".

Gamify them, and pay the best creators so they can work it full time, until some company hires for more them since they are so provenly good.

Destroy useless exams, the only metrics of society are either:

Reduce the entry barrier to education, like Uber has done for taxis.

Help create much greater equal opportunity to talented poor students as described at free gifted education.

Give the students a flexible choice of what to learn, which basically implies that a much large proportion of students get a de-facto gifted education.

In some ways, Ciro wants the website to feel like a video game, where you fluidly interact with headers, comments and their metadata. If game developers can achieve impressively complicated game engines, why can't we achieve a decent amazing elearning website? :-)

Related:

A list of reviews of such systems is maintained at:

This is the class of existing software the perhaps comes the closest to OurBigBook, in particular systems such as:

While we believe that OurBigBook can hold its own against most of them as a personal knowledge base, there is one feature which we believe truly distinguishes OurBigBook from all others in a big way: trustless mind meld with the OurBigBook topic feature, which no other system seems to have.

Many such systems are also no publishing focused enough, and are more focused only in maintaining people's private knowledge bases. Some of them don't even have publishing at all, or its complicated. While publishing is optional in OurBigBook, it is a crucial feature and extremely well supported.

If enough people use it, we could let people sell knowledge content through us.

Teachers have the incentive of making open source to get more students.

Students pay when they want help to learn something.

We take a cut of the transactions.

However this goes a bit against our "open content" ideal.

Forced sponsorware would be a possibility.

Would be a bit like Fiverr. Hmmm, maybe this is not a good thing ;-)

As seen from explicit scalar form of the Maxwell's equations, this expands to 8 equations, so the question arises if the system is over-determined because it only has 6 functions to be determined.

As explained on the Wikipedia page however, this is not the case, because if the first two equations hold for the initial condition, then the othe six equations imply that they also hold for all time, so they can be essentially omitted.

It is also worth noting that the first two equations don't involve time derivatives. Therefore, they can be seen as spacial constraints.

TODO: the electric field and magnetic field can be expressed in terms of the electric potential and magnetic vector potential. So then we only need 4 variables?

Bibliography:

One of the sequencers made by Oxford Nanopore Technologies.

The device has had several updates since however, notably of the pore proteins which are present in the critical flow cell consumable.

Official documentation: nanoporetech.com/products/minion (archive)

The following images of the device and its peripherals were taken during the experiment: Section "How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it".

biochromspectros.com/spectrophotometers/simplinano-cat/simplinano-spectrophotometer.html (archive)

Manual: biochromspectros.com/media/wysiwyg/support_page/support-simplinano/Simplinano-UM.pdf (archive)

The first thing we did was to filter the water samples with a membrane filter that is so fine that not even bacteria can pass through, but water can.

Therefore, after filtration, we would have all particles such as bacteria and larger dirt pieces in the filter.

From the 1 liter in each bottle, we only used 400 ml because previous experiments showed that filtering the remaining 600 ml is very time consuming because the membrane filter gets clogged up.

Therefore, the filtration step allows us to reduce those 400 ml volumes to more manageable Eppendorf tube volumes: Figure 1. "An Eppendorf tube". Reagents are expensive, and lab bench centrifuges are small!

Since the filter is so fine, filtering by gravity alone would take forever, and so we used a vacuum pump to speed thing up!

For that we used:

www.bidspotter.com/en-us/auction-catalogues/bscsur/catalogue-id-bscsur10011/lot-c6605b41-1a14-40e5-a255-a5c5000866e0 (archive) Cannot exact same product on official website, but here is a similar one: www.fishersci.co.uk/shop/products/lm-26-2uv-transilluminator/12382038 (archive).

Biology experiments are hard, and so they go wrong, a lot.

For this reason, it is wise to verify that certain steps are correct whenever possible.

And so this is the first thing we did on the second day!

Gel electrophoresis separates molecules by their charge-to-mass ratio. It is one of those ultra common lab procedures!

This allows us to determine how long are the DNA fragments present in our solution.

Since we know that we amplified the 16S regions which we know the rough size of (there might be a bit of variability across species, but not that much), we were expecting to see a big band at that size.

And that is exactly what we saw!

First we had to prepare the gel, put the gel comb, and pipette the samples into wells present in the gel:

To see the DNA, we added ethidium bromide to the samples, which is a substance that that both binds to DNA and is fluorescent.

Because it interacts heavily with DNA, ethidium bromide is a mutagen, and the biology people sure did treat the dedicated electrophoresis bench area with respect! Figure 4. "Gel electrophoresis dedicated bench area to prevent ethidium bromide contamination.".

The UV transilluminator we used to shoot UV light into the gel was the Fisher Scientific UVP LM-26E Benchtop 2UV Transilluminator. The fluorescent substance then emitted a light we can see.

As barely seen at Figure 8. "Fischer Scientific UVP LM-26E Benchtop 2UV Transilluminator illuminated gel." due to bad photo quality due to lack of light, there is one strong green line, which compared to the ladder matches our expected 16S length. What we saw it with the naked eyes was very clear however.

After filtration, all DNA should present in the filter, so we cut the paper up with scissors and put the pieces into an Eppendorf: Video 1. "Cutting vacuum pump filter and placing it in Eppendorf".

Now that we had the DNA in Eppendorfs, we were ready to continue the purification in a simpler and more standardized lab pipeline fashion.

First we added some small specialized beads and chemicals to the water and shook them Eppendorfs hard in a Scientific Industries Inc. Vortex-Genie 2 machine to break the cell and free the DNA.

Once that was done, we added several reagents which split the solution into two phases: one containing the DNA and the other not. We would then pipette the phase with the DNA out to the next Eppendorf, and continue the process.

In one step for example, the DNA was present as a white precipitate at the bottom of the tube, and we threw away the supernatant liquid: Figure 1. "White precipitate formed with Qiagen DNeasy PowerWater Kit".

At various stages, centrifuging was also necessary. Much like the previous vacuum pump step, this adds extra gravity to speed up the separation of phases with different molecular masses.

In our case, we used a VWR Micro Star 17 microcentrifuge for those steps:

Then, when we had finally finished all the purification steps, we measured the quantity of DNA with a Biochrom SimpliNano spectrophotometer to check that the purification went well:

And because the readings were good, we put it in our -20 C fridge to preserve it until the second day of the workshop and called it a day:

One cool thing we did in this procedure was to use magnetic separation with magnetic beads to further concentrate the DNA: Figure 1. "GE MagRack 6 pipetting.".

The beads are coated to stick to the DNA, which allows us to easily extract the DNA from the rest of the solution. This is cool, but bio people are borderline obsessed by those beads! Go figure!

Then we prepared the DNA for sequencing with the Oxford Nanopore specific part: Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit.

Here some of the steps required a bit more of vortexing for mixing the reagents, and for this we used the VELP Scientifica WIZARD IR Infrared Vortex Mixer which appears to be quicker to use and not as strong as the Vortex Genie 2 previously used to break up the cells:

After all that was done, the DNA of the several 400 ml water bottles and hours of hard purification labour were contained in one single Eppendorf!

This one might actually be understandable! It is what Richard Feynman starts to explain at: Richard Feynman Quantum Electrodynamics Lecture at University of Auckland (1979).

The difficulty is then proving that the total probability remains at 1, and maybe causality is hard too.

The path integral formulation can be seen as a generalization of the double-slit experiment to infinitely many slits.

Feynman first stared working it out for non-relativistic quantum mechanics, with the relativistic goal in mind, and only later on he attained the relativistic goal.

TODO why intuitively did he take that approach? Likely is makes it easier to add special relativity.

This approach more directly suggests the idea that quantum particles take all possible paths.

There are two main ways to try and reach AGI:Which one of them to take is of of the most important technological questions of humanity according to Ciro Santilli

There is also an intermediate area of research/engineering where people try to first simulate the robot and its world realistically, use the simulation for training, and then transfer the simulated training to real robots, see e.g.: realistic robotics simulation.

The quantum NOT gate swaps the state of $|0⟩$ and $|1⟩$, i.e. it maps:As a result, this gate also inverts the probability of measuring 0 or 1, e.g.