Super-resolution means resolution beyond the diffraction limit.
They you can observe fluorophores firing one by one. Their exact position is a bit stochastic and beyond the diffraction limit, but so long as there aren't to many in close proximity, you can wait for it to fire a bunch of times, and the center of the Gaussian is the actual location.
From this we see that super-resolution microscopy is basically a space-time tradeoff: the more time we wait, the better spacial resolution we get. But we can't do it if things are moving too fast in the sample.
Tradeoff with cryoEM: you get to see things moving in live cell. Electron microscopy fully kills cells, so you have no chance of seeing anything that moves ever.
Caveats:
- initial illumination to saturate most fluorophores I think can still kill cells, things get harder the less light you put in. So it's not like you don't kill things at all necessarily, you just get a chance not to
- the presence fluorophore disturbs the system slightly, and is not at the same Exact location of the protein of interest
International Standard Music Number by 
Ciro Santilli 37 Updated 2025-06-02 +Created 1970-01-01
Ciro Santilli 37 Updated 2025-06-02 +Created 1970-01-01Why was this so rarely used as of 2020s compared to ISBNs? It would have been perfect for helping find obscure records from Chinese traditional music and Indian classical music!
Interesting website, hosts mostly:
- datasets
- ANN models
- some live running demos called "apps": e.g. huggingface.co/spaces/ronvolutional/ai-pokemon-card
What's the point of this website vs GitHub? www.reddit.com/r/MLQuestions/comments/ylf4be/whats_the_deal_with_hugging_faces_popularity/
TODO vs Phylogenetic tree? www.visiblebody.com/blog/phylogenetic-trees-cladograms-and-how-to-read-them:
Cladograms and phylogenetic trees are functionally very similar, but they show different things. Cladograms do not indicate time or the amount of difference between groups, whereas phylogenetic trees often indicate time spans between branching points.
A Chess UI is a program that interfaces with a chess engine in order using a computer chess interface to allow human players to interact conveniently with the engine.
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