The DR5 auxin response element is a synthetic regulatory DNA arrangement containing repeated auxin response elements. When used to control a reporter gene, it contributes the responsive regulatory part of a DR5 auxin response reporter. The DNA element and the measurable reporter product are different parts of that system.[ref]
Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements by T. Ulmasov and others. Abstract, Plant Cell9 (1997), pp.1963–1971: synthetic tandem-repeat element and reporter comparisons.
The original study compared DR5 with natural regulatory sequences and found strong auxin-responsive expression in the tested plant systems. It also connected binding by auxin response factor 1 with regulatory effects involving Aux/IAA proteins. This made DR5 useful for studying responsive transcription, rather than establishing a direct physical measurement of auxin concentration.[ref]
Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements by T. Ulmasov and others. Abstract: response comparison, factor binding and repression.
An illuminating experimental distinction is between asking whether a protein recognizes this DNA arrangement and asking whether a reporter is expressed in a particular cell. The first concerns molecular binding; the second also depends on the surrounding regulatory system and reporter behavior. A repeated regulatory element can therefore be a sensitive response tool without being a universal hormone-concentration scale.
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