FRET sensitized emission is FRET acceptor fluorescence caused by transfer from an excited FRET donor. Excite predominantly the FRET donor and collect both emission bands. Reduced FRET donor emission and increased FRET acceptor emission are useful together, but an acceptor-to-donor emission ratio is not automatically the FRET efficiency: brightness, label abundance and channel collection also enter.[ref]
Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM by Hellen C. Ishikawa-Ankerhold and others. §3.4.2: sensitized emission and dependence on optical correction and label concentrations
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