Ciro Santilli @cirosantilli 37

Made up mostly of calcium carbonate.

One cool thing we did in this procedure was to use magnetic separation with magnetic beads to further concentrate the DNA: Figure 1. "GE MagRack 6 pipetting.".

The beads are coated to stick to the DNA, which allows us to easily extract the DNA from the rest of the solution. This is cool, but bio people are borderline obsessed by those beads! Go figure!

Then we prepared the DNA for sequencing with the Oxford Nanopore specific part: Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit.

Here some of the steps required a bit more of vortexing for mixing the reagents, and for this we used the VELP Scientifica WIZARD IR Infrared Vortex Mixer which appears to be quicker to use and not as strong as the Vortex Genie 2 previously used to break up the cells:

After all that was done, the DNA of the several 400 ml water bottles and hours of hard purification labour were contained in one single Eppendorf!

www.qiagen.com/gb/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powerwater-kit (archive) Here is its documentation: www.qiagen.com/gb/resources/download.aspx?id=bb731482-874b-4241-8cf4-c15054e3a4bf&lang=en (archive).

Manual archive: web.archive.org/web/20190911111136/https://www.qiagen.com/gb/resources/download.aspx?id=bb731482-874b-4241-8cf4-c15054e3a4bf&lang=en

Kit to extract clean DNA from water.

www.scientificindustries.com/vortex-genie-2.html (archive)

Cool tool that allows you to graphically visualize page view counts of specific pages. It offers somewhat similar insights to Google Trends.

Homepage: pageviews.wmcloud.org/

Documentation: meta.wikimedia.org/wiki/Pageviews_Analysis#Massviews

The homepage shows views of selected pages, e.g. when Google had their 25th birthday: pageviews.wmcloud.org/?project=en.wikipedia.org&platform=all-access&agent=user&redirects=0&start=2023-09-11&end=2023-10-01&pages=Cat|Dog|Larry_Page Larry Page briefly beat "Cat" and "Dog".

/topviews shows the most viewed pages for a given month: pageviews.wmcloud.org/topviews/?project=en.wikipedia.org&platform=all-access&date=2023-08&excludes= It is extremelly epic that XXX: Return of Xander Cage, a 2017 film, is on the top ten of the August 2023 month. The page was around 8th place on a Google search for "xxx": archive.ph/wip/giRY8 at the time. XXXX (beer) was also on the top 20, followed by Sex on 21.

Java is good.

Its boilerplate requirement is a pain, but the design is otherwise very clean.

But its ecosystem sucks.

The development process is rather closed, the issue tracker obscure.

And above all, Google LLC v. Oracle America, Inc. killed everybody's trust in it once and for all. Thanks Oracle.

Not to be confused with the University of Michigan. Not confusing at all right!

Often just called collimated light due to the collimator being the main procedure to obtain it.

However, you move very far away from the source, e.g. the Sun, you also get essentially parallel light.

Bibliography: