The other major university in the Bay Area (and basically in California itself) besides a few University of California places.
The heart of Silicon Valley.
Not to be confused with the Michigan State University. Not confusing at all right!
Variation of saturation magnetisation with temperature for Nickel
. Source. This graph shows what happens when you approach the Curie temperature from below.They put a lot of emphasis into base calling. E.g.:
- they have used FPGAs to accelerate it on certain models: twitter.com/nanopore/status/841671404588302338, sampe engineer: www.linkedin.com/in/balaji-renganathan-31b98415/
If you live in the relatively food abundant environment of another cell, then you don't have to be able to digest every single food source in existence, of defend against a wide range of predators.
So because DNA replication is a key limiting factor of bacterial replication time, you just reduce your genome to a minimum.
Power, Sex, Suicide by Nick Lane (2006) section "Gene loss as an evolutionary trajectory" puts it well:and also section "How to lose the cell wall without dying" page 184 has some related mentions:
One of the most extreme examples of gene loss is Rickettsia prowazekii, the cause of typhus. [...] Over evolutionary time Rickettsia has lost most of its genes, and now has a mere protein-coding genes left. [...] Rickettsia is a tiny bacterium, almost as small as a virus, which lives as a parasite inside other cells. It is so well adapted to this lifestyle that it can no longer survive outside its host cells. [...] It was able to lose most of its genes in this way simply because they were not needed: life inside other cells, if you can survive there at all, is a spoonfed existence.
While many types of bacteria do lose their cell wall during parts of their life cycle only two groups of prokaryotes have succeeded in losing their cell walls permanently, yet lived to tell the tale. It's interesting to consider the extenuating circumstances that permitted them to do so.[...]One group, the Mycoplasma, comprises mostly parasites, many of which live inside other cells. Mycoplasma cells are tiny, with very small genomes. M. genitalium, discovered in 1981, has the smallest known genome of any bacterial cell, encoding fewer than 500 genes. M. genitalium, discovered in 1981, has the smallest known genome of any bacterial cell, encoding fewer than 500 genes. [...] Like Rickettsia, Mycoplasma have lost virtually all the genes required for making nucleotides, amino acids, and so forth.
Currently an informal name for the Standard Model
Chronological outline of the key theories:
- Maxwell's equations
- Schrödinger equation
- Date: 1926
- Numerical predictions:
- hydrogen spectral line, excluding finer structure such as 2p up and down split: en.wikipedia.org/wiki/Fine-structure_constant
- Dirac equation
- Date: 1928
- Numerical predictions:
- hydrogen spectral line including 2p split, but excluding even finer structure such as Lamb shift
- Qualitative predictions:
- Antimatter
- Spin as part of the equation
- quantum electrodynamics
- Date: 1947 onwards
- Numerical predictions:
- Qualitative predictions:
- Antimatter
- spin as part of the equation
en.wikipedia.org/wiki/Paulo_Freire:OMG so nice.
During his childhood and adolescence, Freire ended up four grades behind, and his social life revolved around playing pick-up football with other poor children, from whom he claims to have learned a great deal. These experiences would shape his concerns for the poor and would help to construct his particular educational viewpoint. Freire stated that poverty and hunger severely affected his ability to learn. These experiences influenced his decision to dedicate his life to improving the lives of the poor: "I didn't understand anything because of my hunger. I wasn't dumb. It wasn't lack of interest. My social condition didn't allow me to have an education. Experience showed me once again the relationship between social class and knowledge"
Power, Sex, Suicide by Nick Lane (2006) paints a colorful picture of the man!
Non formal proof with a program March 2023: www.sligocki.com/2023/03/13/skelet-1-infinite.html Awesome article that describes the proof procedure.
The proof uses Turing machine acceleration to show that Skelet machine #1 is a Translated cycler Turing machine with humongous cycle paramters:
Update multiple rows with different values in a single SQL query Updated 2025-06-17 +Created 1970-01-01
Working remotely is hard if you don't already highly master the software and enterprise systems used.
Also you don't feel people's love as strongly, and usefulness is built on love, see also Steve Jobs's Pixar office space design philosophy.
But please, give workers a small silent office so that we can concentrate instead of a silly open space, and create an internal social network so people can see what others are doing.
Remote working is much better if the majority of the team also does it, otherwise you will get excluded. Maybe after VR...
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it PCR Updated 2025-06-17 +Created 1970-01-01
More generic PCR information at: Section "Polymerase chain reaction".
Because it is considered the less interesting step, and because it takes quite some time, this step was done by the event organizers between the two event days, so participants did not get to take many photos.
PCR protocols are very standard it seems, all that biologists need to know to reproduce is the time and temperature of each step.
This process used a Marshal Scientific MJ Research PTC-200 Thermal Cycler:
We added PCR primers for regions that surround the 16S DNA. The primers are just bought from a vendor, and we used well known regions are called 27F and 1492R. Here is a paper that analyzes other choices: academic.oup.com/femsle/article/221/2/299/630719 (archive) "Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment" by Yuichi Hongoh, Hiroe Yuzawa, Moriya Ohkuma, Toshiaki Kudo (2003)
One cool thing about the PCR is that we can also add a known barcode at the end of each primer as shown at Code 1. "PCR diagram".
This means that we bought a few different versions of our 27F/1492R primers, each with a different small DNA tag attached directly to them in addition to the matching sequence.
This way, we were able to:
- use a different barcode for samples collected from different locations. This means we
- did PCR separately for each one of them
- for each PCR run, used a different set of primers, each with a different tag
- the primer is still able to attach, and then the tag just gets amplified with the rest of everything!
- sequence them all in one go
- then just from the sequencing output the barcode to determine where each sequence came from!
Input: Bacterial DNA (a little bit)
... --- 27S --- 16S --- 1492R --- ...
|||
|||
vvv
Output: PCR output (a lot of)
Barcode --- 27S --- 16S --- 1492RCode 1.
PCR diagram
. Finally, after purification, we used the Qiagen QIAquick PCR Purification Kit protocol to purify the generated from unwanted PCR byproducts.
As mentioned at the prize was claimed at 8d31992805518fd62daa3bdd2a5c4fd2cd3054c9b3dca1d78055e9528cff6adc (2017-02-23) which spends several inputs with the same unlock script that presents two different constantants that have the same SHA-1:both givingIt was claimed on the same day that Google disclosed the collision: security.googleblog.com/2017/02/announcing-first-sha1-collision.html
printf 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 | xxd -r -p | sha1sum
printf 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 | xxd -r -p | sha1sumf92d74e3874587aaf443d1db961d4e26dde13e9cBoth of these are PDF prefixes, so they start with the PDF file signature, but are not fully viewable PDFs on their own.
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