 Solution

In gel sieving of synthetic polymers, gel electrophoresis combines drift under an electric field with size- and conformation-dependent obstruction by a porous matrix. In weak-field free solution, force balance gives $v=\mu E$ and, in a simple effective-charge/effective-friction model, $\mu =q_{\mathrm{eff}}/\zeta$. The mobility is motion relative to the medium, so fluid motion from electro-osmosis must be accounted for separately. This is the operational mobility convention, not a claim that the bare structural charge is always the effective driving charge.^[ref]

For a chain with free-draining polymer friction with approximately constant effective electric charge per segment, both charge and friction scale with $N$. The free-solution mobility can therefore become nearly independent of length, making size separation poor. A gel adds steric constraints. Coils smaller than the mesh can pass relatively easily; larger coils require deformation and pore-to-pore rearrangement. A schematic weak-sieving law is $\mu (c_g)\simeq \mu (0)e^{-K_r{c}_g}$, where matrix concentration $c_g$ and retardation coefficient $K_r$ are defined by a calibration; it is not a universal size law.

When a long flexible charged polymer is confined to a tube of available paths, reptation supplies another regime. An additive-friction model gives contour diffusion $D_c\propto N^{-1}$ and renewal time $\tau \sim N^2/D_c\propto N^3$. For ideal large-scale chain statistics, $R^2\propto N$, so centre-of-mass diffusion scales as $N^{-2}$. With total effective driving charge proportional to $N$, weak-field mobility can then scale as $N^{-1}$. This assumes screened/ideal large-scale conformations and linear response; strong-field alignment, trapping and nonideal matrices can alter the scaling.

Operationally, load narrow zones of independently characterized synthetic standards and sample into a controlled gel, apply a known field and detect migration positions after a measured time. Compare with a free-solution measurement, vary matrix concentration and field, and control temperature, ionic strength, pH and matrix charge. Larger gel pores or lower matrix concentration usually reduce sieving within a given calibrated material system. Excessive field causes Joule heating and can change conformation and mobility. adsorption or electro-osmotic drift can mimic a size effect; standards should match charge chemistry and topology as well as nominal mass.

Band centres move as $\mu Et$ while ordinary diffusion gives ${\sigma }_x^2(t)={\sigma }_x^2(0)+2Dt$. Resolution depends on separation of centres relative to band widths, not travel distance alone. A neutral tracer helps identify bulk flow, and multiple concentrations test adsorption or overloading. A mobility or band position does not uniquely determine size without a regime-specific calibration. TODO: biomolecular examples, sample preparation and biological applications are user deferred; the account here concerns synthetic charged polymers only.