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A holographic object wave is the object-dependent field arriving at the recording surface, including the propagation from the object. Its complex amplitude generally differs from the transmission function at the object itself. Distinguishing these two planes is essential when refocusing a hologram.

A holographic reference wave is a known coherent comparison field at the recording surface. Its wave phase supplies the standard against which the holographic object wave is encoded. In in-line holography, the undiffracted part of the illumination can supply this field; in off-axis holography, a separate tilted beam supplies it.

Holography records the wave interference between a holographic object wave and a known holographic reference wave, then uses that record to reconstruct a wavefront. The record is a hologram. A photographic plate or photodetector measures light intensity; the relative wave phase is encoded in the positions and contrast of interference fringes, rather than measured directly as an optical oscillation. Reillumination makes the recorded pattern act as a diffraction grating that produces an image-bearing field.

The useful change of viewpoint is to preserve a field that can propagate again, instead of only a focused brightness picture. Recovered complex amplitude permits refocusing with the Fresnel propagator; a spatially extended hologram can also supply different viewing directions. The recoverable information is limited by the collected aperture, recorded spatial frequencies, optical coherence and separation of unwanted diffraction orders.

In-line holography uses nearly the same direction for the holographic reference wave and holographic object wave. In a transmission geometry, the undiffracted illumination supplies $R$ while the object supplies the scattered component $O$. This makes small optical path differences possible, but leaves the desired image, its twin image in holography and the bright background sharing an axis.

Dennis Gabor's electron-microscopy motivation led to optical tests: one line of a high-pressure mercury-vapor lamp illuminated a 3 μm pinhole, then approximately 1 mm microphotographs, recording approximately 1 cm holograms on sensitive photographic emulsions with minute-scale exposures. The roughly 0.1 mm optical coherence length favoured an in-line arrangement. Reillumination reconstructed images; focusing separated their depths incompletely. Distorted letters and random optical disturbances limited the results. These were optical demonstrations, not an operating electron microscope.^[ref]

The recorded microphotograph and its reconstructed lettering provide an object-to-image comparison in that account. The blocked-reference control described above is an explanatory control for the model; it is not a claim about an additional historical measurement.

Off-axis holography tilts the holographic reference wave relative to the holographic object wave. Its interference fringes acquire a carrier spatial frequency; the two cross terms occupy different spatial frequency regions when the carrier is sufficiently large compared with the object's signal bandwidth. This permits filtering a single image term.

Gabor's lecture credits Emmett Leith and Juris Upatnieks with the later skew-reference method using helium–neon laser illumination, separating reconstructed images angularly.^[ref]

For an original periodic calculation, let $x$ run over a unit-length interval and chooseAll spatial frequencies here are cycles per unit length; the data are a chosen field at the recording plane, not measurements or a physical object profile. Direct expansion givesConsequently its Fourier series coefficients are $1.05$ at zero, $0.02$ at $\pm 8$, $0.2$ at $\pm 17$ and $0.1$ at $\pm 25$. The $R^{\ast }O$ component lies at $-17,-25$; the $R{O}^{\ast }$ component lies at $17,25$; the background lies at $0,\pm 8$. Filtering the negative-frequency cross term and multiplying by $R$ recovers $O$, including its relative wave phase.

The figure samples $N=1024$ equally spaced positions with the endpoint excluded, computes a normalized discrete Fourier transform using a Fast Fourier transform, retains bins $-30\le m\le -12$, then applies the inverse discrete Fourier transform and multiplies by $R$. The recovered real and imaginary parts coincide with the chosen $O$ to numerical precision. This exact periodic example avoids leakage by placing every frequency on an integer bin; finite nonperiodic records need allowance for broadening and an aperture.

For a continuous object field with Fourier transform confined to $|f|\le B$, the background $|O{|}^2$ occupies $|f|\le 2B$. A carrier $f_c>3B$ is a sufficient separation condition for the sideband centred at $-f_c$, and $f_c+B$ must remain below the Nyquist spatial frequency. Here $B=5$, $f_c=20$ and the Nyquist spatial frequency is $512$. This sufficient bound describes a whole bandwidth, rather than exploiting the gaps in this particular two-component field.

The same example also makes optical playback explicit. Choose $t_0=0.3$ and $\beta =0.01$. Since $|O|\le 0.3$, the bounds $0.49\le I\le 1.69$ imply $0.3049\le t\le 0.3169$, and $|\beta I|/t_0\le 0.0564$. The affine response is stipulated; its weak-modulation interpretation is approximate. In $Rt$, the desired field $0.01O$ has frequencies $3,-5$; the conjugate term $0.01R^2{O}^{\ast }$ has frequencies $37,45$; the background has frequencies $20,12,28$. Thus the same carrier that separates the recorded cross terms separates the playback components. Reillumination restores a field through diffraction; computational filtering extracts the corresponding encoded field through Fourier analysis.