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Osmotic lysis is rupture after excessive water entry and swelling. It is an outcome for susceptible biological cells, not an inevitable response of every biological cell to a hypotonic solution. Cell walls can bear the resulting load, and cellular mechanisms can regulate water balance. A protoplast challenge tests a particular preparation under particular conditions.

A hypotonic solution favors initial net water entry under the reference conditions above. A susceptible biological cell may undergo osmotic lysis; a sufficiently strong cell wall can instead support turgor pressure.

A permeating solute can cross the specified membrane appreciably during the observation interval. This is a property of a solute-membrane-time comparison, not an immutable label attached to a molecule. Passage can reduce a concentration difference through passive transport while water is also moving; it does not require water to wait.

For an original dilute ideal example, stipulate a flexible model biological cell with $20\mathrm{mmol}{\mathrm{L}}^{-1}$ retained particles and an external reservoir with $10\mathrm{mmol}{\mathrm{L}}^{-1}$ retained particles plus $10\mathrm{mmol}{\mathrm{L}}^{-1}$ freely permeating neutral solute. Initially the total osmolarities are equal. Suppose the permeating solute eventually has equal internal and external concentrations, there is negligible pressure difference, no active transport, and the internal retained particle amount is conserved. The permeating contributions cancel from the final osmotic balance; the internal retained concentration must fall from $20$ to $10\mathrm{mmol}{\mathrm{L}}^{-1}$. The required volume is twice its initial value. This illustrates an initially equal-osmolarity but hypotonic solution; it assumes the boundary can survive that expansion and predicts no universal time course.

Tonicity describes how a surrounding chemical solution changes cellular volume through osmosis. It depends on which solutes remain effectively excluded on the timescale of interest, not simply on the amount of dissolved material. The following comparisons take a functional cell membrane, an initially unpressurized biological cell, and effectively excluded solutes as the reference; rising turgor pressure can oppose entry in a walled biological cell.

Operationally, hypotonic solutions cause sustained swelling, hypertonic solutions sustained shrinking, and isotonic solutions no sustained volume change in the specified passive comparison. State the reference biological cell, membrane properties and observation interval. A permeating solute can transiently change water potential while crossing; after its chemical potential equilibrates, its initial unequal concentrations cannot be treated as a permanently retained osmotic load. Thus equal osmolarities need not imply isotonic solutions. Conversely, a stationary walled biological cell may have unequal osmolarities balanced by turgor pressure.^[ref]