Past exam of the mathematics and the environment and mathematical physiology course of the University of Oxford / 2008 / B / 5 / i / Solution

For an assay begun by mixing free enzyme and substrate, take $\boxed{[S](0)=S_0>0,[E](0)=E_0>0,[C](0)=[P](0)=0}$. The complex has not yet formed and no product has been made. The later small-enzyme limit assumes $E_0\ll S_0$. Other preincubation protocols could require nonzero initial complex, but these initial conditions are the ones used by the stated inner solution.