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In 1992 the COBE DMR team, including George F. Smoot and Charles L. Bennett, used radiometer antennas to compare antenna temperatures between sky directions $60^{\circ }$ apart at $31.5$, $53$ and $90\mathrm{GHz}$, with two nearly independent channels per frequency. Repeated sky coverage supported least-squares solution map reconstruction.^[ref]

The analysis fitted magnetic response and removed the assumed kinematic angular dipole and its second-order Doppler effect contribution. Channel sums retained sky structure; channel differences estimated noise. Cross-channel correlations, different frequency maps and Milky Way latitude cuts tested the interpretation. Residual large-angle structure supported Cosmic microwave background anisotropy; Galactic contamination particularly limited the cosmic microwave background quadrupole. Brightness fluctuations alone were not sufficient evidence of primordial structure.^[ref]

The two differences serve different purposes. Comparing two sky directions measures angular variation. Comparing two independently calibrated receiver maps observing the same sky tests noise and consistency. A common sky signal cancels in the latter comparison if their antenna beam patterns and response scales match; a systematic error shared by both channels need not cancel. Separating observer motion, local emission and intrinsic Cosmic microwave background anisotropy requires these additional tests.

The cosmic microwave background is nearly isotropic thermal electromagnetic radiation. Its spectrum connects radio receiver measurements with the universe's thermal history: redshift preservation of a thermal photon spectrum cools a blackbody spectrum as space expands. The 1965 Princeton analysis described this relation.^[ref]

A radiometer cannot label a received photon as cosmic, atmospheric or instrumental. Its measured power combines the incoming radiation with the receiver response. The useful question is which comparisons and controls can isolate a sky contribution. This makes the Penzias-Wilson microwave background experiment an example of both observational cosmology and measurement calibration.

In 1965, Arno Penzias and Robert Woodrow Wilson reported measurements with a 20-foot horn-reflector antenna at $4.080\mathrm{GHz}$. They manually switched a traveling-wave maser receiver between the horn and a helium-cooled reference. From $6.7\mathrm{K}$ zenith antenna temperature, atmospheric and antenna/ground contributions left an excess $3.5\pm 1.0\mathrm{K}$.^[ref]

Elevation scans estimated the atmospheric term. Taping horn seams produced no measurable change; a nearby transmitter tested ground response. These controls addressed distinct possible contaminations.^[ref]

The comparison does not make all nuisance contributions disappear. A reference can establish a response scale, but atmospheric emission and pickup through unwanted antenna directions are also real incident radiation. In his later account, Robert Woodrow Wilson describes these competing contributions as central to a radio astronomy measurement.^[ref]

The Princeton group proposed primordial thermal radiation as an interpretation and called for more wavelength measurements. Their separate $3\mathrm{cm}$ radiometer had not yet produced results.^[ref] A single-frequency excess therefore does not by itself establish a complete Planck spectrum. Reproducibility across independent instruments, observing directions and frequencies tests the interpretation further.

A radiometer measures electromagnetic radiation through its received power. A radio radiometer can express that power as a noise temperature, allowing an unknown source to be compared with calibrated reference temperatures.

For a stable radiometer with affine output $S=g(T+T_r)$, let $g>0$ be the fixed response coefficient and $T_r$ the receiver noise temperature. Two reference loads with known equivalent input noise temperatures $T_c<T_h$ give outputs $S_c$ and $S_h$. Subtracting their outputs eliminates $T_r$; dividing source and reference differences eliminates $g$:This two-load radiometer calibration assumes unchanged response and receiver noise temperature between readings, with the loads and source referred to the same input. It also eliminates an additional constant output offset. Physical load temperatures must first be converted to the applicable equivalent input noise temperatures when the classical thermal approximation fails. The Penzias-Wilson microwave background experiment gives an original worked example.