Binds an amino acid to the correct corresponding tRNA sequence. Wikipedia mentions that humans have 20 of them, one for each proteinogenic amino acid.
If either PAE and PSE are active, different paging level schemes are used:
- no PAE and no PSE:
10 | 10 | 12 - no PAE and PSE:
10 | 22.22 is the offset within the 4Mb page, since 22 bits address 4Mb. - PAE and no PSE:
2 | 9 | 9 | 12The design reason why 9 is used twice instead of 10 is that now entries cannot fit anymore into 32 bits, which were all filled up by 20 address bits and 12 meaningful or reserved flag bits.The reason is that 20 bits are not enough anymore to represent the address of page tables: 24 bits are now needed because of the 4 extra wires added to the processor.Therefore, the designers decided to increase entry size to 64 bits, and to make them fit into a single page table it is necessary reduce the number of entries to 2^9 instead of 2^10. - PAE and PSE:
2 | 9 | 21
Study of the metabolome.
The Linux Kernel reserves two zones of virtual memory:
- one for kernel memory
- one for programs
The exact split is configured by
CONFIG_VMSPLIT_.... By default:- on 32-bit:
- on 64-bit: currently only 48-bits are actually used, split into two equally sized disjoint spaces. The Linux kernel just assigns:
- the bottom part to processes
00000000 00000000to008FFFFF FFFFFFFF - the top part to the kernel:
FFFF8000 00000000toFFFFFFFF FFFFFFFF, like this:------------------ FFFFFFFF Kernel ------------------ C0000000 (not addressable) ------------------ BFFFFFFF Process ------------------ 00000000
- the bottom part to processes
Kernel memory is also paged.
In previous versions, the paging was continuous, but with HIGHMEM this changed.
There is no clear physical memory split: stackoverflow.com/questions/30471742/physical-memory-userspace-kernel-split-on-linux-x86-64
Technique widely used to measure the size of DNA strands, most often PCR output of a region of interest.
A simple sample application is gel electrophoresis alelle determination.
zettelkasten.de/posts/overview/ mentions one page to rule them all:
How many Zettelkästen should I have? The answer is, most likely, only one for the duration of your life. But there are exceptions to this rule.
"De novo" means "starting from scratch", that is: you type the desired sequence into a computer, and the synthesize it.
The "de novo" part is important, because it distinguishes this from the already well solved problem of duplicating DNA from an existing DNA template, which is what all our cells do daily, and which can already be done very efficiently in vitro with polymerase chain reaction.
Notably, the dream of most of those companies is to have a machine that sits on a lab bench, which synthesises whatever you want.
The initial main applications are likely going to be:but the real pipe dream is building and bootstraping entire artificial chromosomes
- polymerase chain reaction primers (determine which region will be amplified
- creating a custom sequence to be inserted in a plasmid, i.e. artificial gene synthesis
News coverage:
Nuclera eDNA enzymatic de novo DNA synthesis explanatory animation (2021)
Source. The video shows nicely how Nuclera's enzymatic DNA synthesis works:- they provide blocked nucleotides of a single type
- add them with the enzyme. They use a werid DNA polymerase called terminal deoxynucleotidyl transferase that adds a base at a time to a single stranded DNA strand rather than copying from a template
- wash everything
- do deblocking reaction
- and then repeat until done
Pinned article: Introduction to the OurBigBook Project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 3. Visual Studio Code extension installation.
Figure 4. Visual Studio Code extension tree navigation.
Figure 5. Web editor. You can also edit articles on the Web editor without installing anything locally.Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension.Video 4. OurBigBook Visual Studio Code extension editing and navigation demo. Source. 
- Infinitely deep tables of contents:
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact