Like PCR, but does not require thermal cycling. Thus the "isothermal" in the name: iso means same, so "same temperature".

Not needing the thermo cycling means that the equipment needed is much smaller and cheaper it seems.

Trade-offs question: biology.stackexchange.com/questions/92172/what-are-the-trade-offs-between-polymerase-chain-reaction-pcr-vs-loop-mediated

Caused by slipped strand mispairing.

oriC = Origin of Chromosomal replication.

Most of these are going to be Whole-genome sequencing of some model organism:en.wikipedia.org/wiki/Whole_genome_sequencing#History lists them all. Basically th big "firsts" all happened in the 1990s and early 2000s.

Can be seen as a cheap form of DNA sequencing that only test for a few hits. Some major applications:

Experiments that involve sequencing bulk DNA found in a sample to determine what species are present, as opposed to sequencing just a single specific specimen. Examples of samples that are often used:

One related application which most people would not consider metagenomics, is that of finding circulating tumor DNA in blood to detect tumors.

Sequencing the DNA tells us what the organism can do. Sequencing the RNA tells us what the organism is actually doing at a given point in time. The problem is not killing the cell while doing that. Is it possible to just take a chunk of the cell to sequence without killing it maybe?

The by far dominating DNA sequencing company of the late 2000's and 2010's due to having the smallest cost per base pair.

Illumina actually bought their 2010's dominating technology from a Cambridge company called Solexa.

To understand how Illumina's technology works basically, watch this video: Video 1. "Illumina Sequencing by Synthesis by Illumina (2016)".

The key innovation of this method is the Bridge amplification step, which produces a large amount of identical DNA strands.

This is one of the the key innovations of the Illumina (originally Solexa) sequencing.

This step is genius because sequencing is basically a signal-to-noise problem, as you are trying to observe individual tiny nucleotides mixed with billions of other tiny nucleotides.

With bridge amplification, we group some of the nucleotides together, and multiply the signal millions of times for that part of the DNA.

They put a lot of emphasis into base calling. E.g.:

Red clump stars are a specific type of red giant star in the later stages of stellar evolution. These stars are typically in the horizontal branch phase, which follows the giant phase of a star's life cycle. Red clump stars represent a phase where stars have exhausted the hydrogen in their cores and are now fusing helium into carbon and oxygen. They are somewhat hotter and brighter than ordinary red giants, and their spectra show strong absorption lines indicative of helium fusion.

Exoplanets detected by radial velocity, also known as the Doppler method or the radial velocity method, is a technique used to identify exoplanets by observing the gravitational influence they have on their host stars. This method takes advantage of the Doppler effect, where the light emitted by a star shifts in wavelength depending on its motion relative to an observer.

Collisional excitation is a process in which an atom or molecule absorbs energy during a collision with a particle, such as another atom, molecule, or electron. This energy transfer can promote an electron within the atom or molecule to a higher energy state, or excited state. Here's how it works: 1. **Encounter**: During a collision, kinetic energy from the colliding particle (which can be a gas particle or an electron) is transferred to the target atom or molecule.