www.biorxiv.org/content/10.1101/2022.09.19.508583v1.fullIt is also interesting to see how they are interested in co-culture with HeLa cells, presumably to enable infectious bacterial disease studies.
CVI-syn3B strains differ from JCVI-syn3.0 by the presence of 19 additional non-essential genes that result in a more easily manipulated cell. JCVI-syn3B additionally includes a dual loxP landing pad that enables easy Cre recombinase mediated insertion of genes
At biology.indiana.edu/news-events/news/2023/lennon-minimal-cells.html (2023) they let it re-evove to it it would regain some fitness, and it did.
A report on the Navier-Stokes Problem by Vladimir Šverak
. Source. 2025.en.wikipedia.org/w/index.php?title=Nutrient&oldid=1075972831#Essential gives somewhat of an overview:
20 minutes in optimal conditions, with a crazy multiple start sites mechanism: E. Coli starts DNA replication before the previous one finished.
Otherwise, naively, would take 60-90 minutes just to replicate and segregate the full DNA otherwise. So it starts copying multiple times.
- biology.stackexchange.com/questions/30080/how-can-e-coli-proliferate-so-rapidly
- stochasticscientist.blogspot.co.uk/2012/02/how-e-coli-grows-so-fast.html
- www.ncbi.nlm.nih.gov/pmc/articles/PMC2063475/ Organization of sister origins and replisomes during multifork DNA replication in Escherichia coli by Fossum et al (2007)
Some people from them contacted Ciro Santilli after Ciro's initial publishing of CIA 2010 covert communication websites.
After a quick Discord chat with them, it was apparent that these people were really cool and knowledgeable.
Also many of them seem to think university is broken and just go hack straigh away.
Also they don't seem to need sleep. Go figure!
With pepole like this, there's hope for Brazil: Section "What poor countries have to do to get richer".
Ciro Santilli has become slightly obsessed with this story, and the main mastermind Ross Ulbricht.
The best article available so far is: www.theregister.co.uk/2019/01/29/how_i_caught_silk_road_mastermind (archive) which summarizes what one of the investigators said in a 2019 French computer security conference.
The key living posts are:
- stackoverflow.com/questions/15445285/how-can-i-connect-to-a-tor-hidden-service-using-curl-in-php (archive) which was originally asked under the real name, and then the username was changed to "Frosty", which matches one of the server's logins after the laptop was captured
- altoid early Silk Road mention: bitcointalk.org/?topic=175.70;wap2 (archive)
The big question is of course how libertarian free market ideologically motivated the website was, and how purely criminal greed it was.
The magnitude of the early operational security mistakes does make Ciro think that Ross did it "because he could" and "for the lolz" in a real world Breaking Bad way.
The entry in Ross' diary does resonate a lot with Ciro and any entrepreneur, full diary at: www.wired.com/2015/01/heres-secret-silk-road-journal-laptop-ross-ulbricht/ (archive).
[i]n 2011," [I believe I will be] "creating a year of prosperity and power beyond what I have ever experienced before,Silk Road is going to become a phenomenon and at least one person will tell me about it, unknowing that I was its creator."
Having this kind of feeling, is the greatest thing any human can have, and what motivates all great things.
Capitalizing in illegal things though is a cheat, big things take longer than a few years to reach, but reaching them is that much more satisfying as well.
Other interesting quotes:which Ciro also feels, see don't be a pussy, and:
Everyone knows I am working on a bitcoin exchange. I always thought honesty was the best policy and now I didn't know what to do. I should have just told everyone I am a freelance programmer or something, but I had to tell half truths. It felt wrong to lie completely so I tried to tell the truth without revealing the bad part, but now I am in a jam. Everyone knows too much. Dammit.
Also very worth reading is the San Francisco flat mate account: www.vice.com/en_us/article/ae3q8g/my-roommate-the-darknet-drug-lord (archive).
The murder for hire allegations are also interesting: mashable.com/2013/10/03/silk-road-hits, he paid 80k dollars to undercover DEA agents!
Except for the fact that Ross was an 80 million Dollar drug lord, those accounts sound exactly like what you would expect from any other nerdy startup founder! The:
- "just do it" strategy effectively going to a minimal viable product (manual transaction management!), while making many mistakes along the way, including hiring mistakes and successes when scaling is needed
- the hardship of self bootstrapping your own social network (here with some kilos of mushrooms)
- the variety of periods, from relatively calm, to hair pulling stress during big changes
It is also amusing to see very concretely the obvious fact that the FBI can get a subpoena for all accounts you ever had, e.g. they knew his laptop model from Amazon and brought a corresponding power cable to the arrest! If you are going to be a cyber criminal, don't use your real name, ever!
Should justice be blind? Maybe. But it does hurt for mere non-blind men to see it sometimes. Especially when drug liberalization is involved.
Notation used in quantum mechanics.
Ket is just a vector. Though generally in the context of quantum mechanics, this is an infinite dimensional vector in a Hilbert space like .
Bra is just the dual vector corresponding to a ket, or in other words projection linear operator, i.e. a linear function which can act on a given vector and returns a single complex number. Also known as... dot product.
For example:is basically a fancy way of saying:that is: we are taking the projection of along the direction. Note that in the ordinary dot product notation however, we don't differentiate as clearly what is a vector and what is an operator, while the bra-ket notation makes it clear.
The projection operator is completely specified by the vector that we are projecting it on. This is why the bracket notation makes sense.
It also has the merit of clearly differentiating vectors from operators. E.g. it is not very clear in that is an operator and is a vector, except due to the relative position to the dot. This is especially bad when we start manipulating operators by themselves without vectors.
This notation is widely used in quantum mechanics because calculating the probability of getting a certain outcome for an experiment is calculated by taking the projection of a state on one an eigenvalue basis vector as explained at: Section "Mathematical formulation of quantum mechanics".
Making the projection operator "look like a thing" (the bra) is nice because we can add and multiply them much like we can for vectors (they also form a vector space), e.g.:just means taking the projection along the direction.
Ciro Santilli thinks that this notation is a bit over-engineered. Notably the bra's are just vectors, which we should just write as usual with ... the bra thing makes it look scarier than it needs to be. And then we should just find a different notation for the projection part.
Maybe Dirac chose it because of the appeal of the women's piece of clothing: bra, in an irresistible call from British humour.
But in any case, alas, we are now stuck with it.
Pinned article: Introduction to the OurBigBook Project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 3. Visual Studio Code extension installation.
Figure 4. Visual Studio Code extension tree navigation.
Figure 5. Web editor. You can also edit articles on the Web editor without installing anything locally.Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension.Video 4. OurBigBook Visual Studio Code extension editing and navigation demo. Source. 
- Infinitely deep tables of contents:
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact