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A. M. de Vos investigated how growth hormone binds its growth hormone receptor. With colleagues, de Vos determined the X-ray crystallography structure of a complex containing human growth hormone and the extracellular portion of its receptor. The 1992 structure, measured at $2.8\text{A˚}$, contained one hormone molecule and two receptor molecules.^[ref]

The growth hormone molecule contacted the two receptors through different binding surfaces, although the receptors used largely the same residues in each contact. Additional contact between the two receptors supported a model in which hormone binding brings a receptor pair together. This made the geometry of signal transduction concrete: a single bound hormone can organize more than one receptor molecule. The measured complex contained extracellular fragments, so the structure did not by itself record the complete activation process across a cell membrane.^[ref]

Growth hormone is a protein hormone synthesized and released by the anterior pituitary. It regulates body growth and metabolism, acting on tissues directly and partly through insulin-like growth factor 1 produced by the liver and other tissues. Its release is controlled by signals from the hypothalamus, including inhibition by somatostatin.^[ref]

This makes “growth” a coordinated physiological response rather than merely a change in one chemical concentration. Growth hormone can initiate signaling in one tissue, while a second circulating signal influences other tissues. Negative feedback connects the resulting signal levels back to their control pathway.^[ref]

A revealing molecular experiment determined the structure of human growth hormone bound to the extracellular portions of the growth hormone receptor. The 2.8-angstrom X-ray crystallography structure contained one hormone molecule and two receptor portions. Two different surfaces of the hormone contacted the receptor partners. This provides a concrete example of a signaling molecule organizing a multi-part complex, rather than simply occupying one isolated pocket. The structure supported a model for receptor association; establishing the full sequence of activation in a living biological cell requires additional functional experiments.^[ref]

The growth hormone receptor is a membrane receptor that recognizes growth hormone and couples its binding to intracellular signaling through the associated tyrosine kinase JAK2. The receptor binds the extracellular hormone, while JAK2 supplies a catalytic signaling activity on the cytoplasmic side. Binding and kinase activation are related but distinct events.^[ref]

A 1992 crystal structure resolved one growth hormone molecule bound to two receptor extracellular domains at 2.8-angstrom resolution. The hormone contacted the two receptor molecules through different surfaces. This establishes an informative stoichiometry and geometry, but the isolated extracellular complex does not by itself show all movements or phosphorylation reactions in a living membrane.^[ref]

Later experiments tested the geometry inside receptor dimers. A 2014 study used Förster resonance energy transfer to monitor the relative positions of receptor motifs that bind JAK2, together with engineered receptors and molecular modeling. The resulting model linked receptor rearrangement to changes in how kinase and inhibitory pseudokinase regions interact.^[ref]

An important consequence is that measuring hormone occupancy alone cannot specify the signaling output. Two receptor configurations can have bound ligand yet position their intracellular partners differently. Experiments that separately measure binding, geometry and downstream kinase activity are therefore more informative than assuming that every occupied receptor is equally active.