Intrinsic termination refers to a process in molecular biology, particularly in the context of transcription, where RNA synthesis is terminated naturally without the need for additional factors or signals. This type of termination occurs when the RNA polymerase encounters specific sequences in the DNA that induce the formation of a stable structure in the newly synthesized RNA molecule, leading to the release of the RNA transcript.
KIF1C is a gene that encodes a member of the kinesin family of motor proteins. Kinesins are involved in intracellular transport, and they move along microtubules, which are part of the cytoskeleton in eukaryotic cells.
Mycofactocin is a relatively recently identified metabolite produced by certain bacteria, particularly those in the phylum Actinobacteria, including some mycobacterial species. It is thought to play a role in the response of these bacteria to oxidative stress and may also be implicated in other cellular processes. Mycofactocin is known to be involved in the redox biology of cells, which includes the regulation of oxidative states and response to reactive oxygen species (ROS).
Nested polymerase chain reaction (nested PCR) is a modification of the standard polymerase chain reaction (PCR) technique that is designed to increase the specificity and sensitivity of the amplification of a target DNA sequence. It involves two consecutive rounds of PCR using two sets of primers. ### Steps Involved in Nested PCR: 1. **First Round of PCR**: - The first set of primers (outer primers) is used to amplify a larger target region of the DNA.
Pdr1p (Pleiotropic Drug Resistance 1 protein) is a regulatory protein found in certain yeast, particularly in *Saccharomyces cerevisiae* (baker's yeast). It is a transcription factor that plays a crucial role in the cellular response to various drugs and toxic compounds. Pdr1p is involved in the regulation of a number of genes associated with multidrug resistance, which helps the yeast cell to survive in the presence of potentially harmful substances.
Pyrosequencing is a DNA sequencing technique that involves the detection of pyrophosphate release during the DNA synthesis process. It is a type of sequencing by synthesis method that allows for real-time monitoring of the incorporation of nucleotides. Here’s how it works: 1. **Template Preparation**: A single-stranded DNA template is created, which serves as a template for sequencing.
Sequencing generally refers to the process of determining the order of elements in a particular context. The specific meaning of sequencing can vary widely depending on the field in which it is used. Here are a few common contexts in which the term "sequencing" is applied: 1. **Genetics**: In genetics, sequencing refers to determining the exact order of nucleotides in a DNA or RNA molecule.
Transfection is a laboratory technique used to introduce nucleic acids—such as DNA or RNA—into cells. This process is often employed in molecular biology and genetics research for various purposes, including: 1. **Gene Expression Studies**: Researchers can introduce genes into cells to study how they function and what effects they have on cellular processes. 2. **Protein Production**: Transfected cells can produce proteins, which can then be purified for research or therapeutic purposes.
In molecular biology, a **vector** is a DNA molecule used as a vehicle to transfer genetic material into a host cell. Vectors are essential tools in genetic engineering, cloning, and various biotechnological applications. They can carry genes of interest, facilitating the introduction of these genes into cells for various purposes, such as gene expression, gene therapy, or the production of proteins.
The Warburg hypothesis, proposed by the German biochemist Otto Warburg in the 1920s, suggests that cancer cells primarily rely on aerobic glycolysis for energy production, even in the presence of sufficient oxygen, which is different from normal cells that typically generate energy through oxidative phosphorylation in the mitochondria. This phenomenon, known as the "Warburg effect," indicates that cancer cells preferentially convert glucose to lactate instead of fully oxidizing it to carbon dioxide and water.
ZMapp is an experimental therapeutic drug that was developed for the treatment of Ebola virus disease. It is a type of monoclonal antibody therapy, which means it is designed to target and neutralize the Ebola virus in the body. ZMapp was created by combining three different monoclonal antibodies that can bind to the virus and help the immune system fight off the infection.
Indian fluid dynamicists are scientists and engineers from India who specialize in the study of fluid mechanics, which is the branch of physics that deals with the behavior of fluids (liquids and gases) in motion and at rest. Fluid dynamics is crucial in various fields, including aerodynamics, hydrodynamics, meteorology, and engineering applications like the design of aircraft, automobiles, and hydrodynamic systems. India has a rich history of contributions to fluid dynamics through both theoretical work and practical applications.
Lu Shijia, also known as "Luzhijia," is a type of traditional Chinese cuisine, particularly known in the Jiangsu and Zhejiang provinces of China. The focus of Lu Shijia cuisine is on fresh ingredients, delicate flavors, and beautiful presentation, often featuring seafood, seasonal vegetables, and techniques like steaming and braising.
Akiva Yaglom is a prominent figure in the field of mathematics and mathematical physics, particularly known for his contributions to the areas of geometry, mathematical physics, and applied mathematics. He was a Soviet mathematician who made significant advances in the study of geometric structures and their applications. One of his notable works involved the development of various geometric methods and their implications in physics, particularly in understanding concepts related to relativity and quantum mechanics.
Gallon per watt-hour (gal/Wh) is a unit of measurement that expresses the quantity of energy produced or consumed in relation to the volume of fuel used. Specifically, it measures how many gallons of fuel are needed to generate one watt-hour of electrical energy. This metric can be particularly useful in evaluating the efficiency of power generation systems, especially those that rely on liquid fuels, such as gasoline or diesel generators.
Mariolina Padula is an Italian artist known for her work in sculpture and installation art. Her pieces often explore themes of intimacy, memory, and the human experience, using natural materials and organic forms.
Jurimetrics is an interdisciplinary field that applies quantitative and statistical methods to legal problems and issues. It combines elements of law, mathematics, statistics, and computer science to analyze legal data and facilitate legal decision-making. Jurimetrics can include the use of data analysis to study legal trends, predict outcomes of legal cases, evaluate the effectiveness of laws, and improve legal processes.
Paul Richard Heinrich Blasius (1883-1970) was a German engineer and physicist, best known for his contributions to fluid dynamics and aerodynamics. One of his significant achievements is the Blasius solution, which describes the flow of a viscous fluid over a flat plate, providing important insights into boundary layer theory. His work laid important foundations for understanding laminar flow and the behavior of fluids in various engineering applications.
R. Cengiz Ertekin is a prominent figure in the field of engineering and academia, particularly known for his contributions to mechanical engineering and fluid mechanics. He has published numerous research papers and has been involved in various educational initiatives, often holding significant positions in academic institutions.
Samar Mubarakmand is a Pakistani nuclear scientist known for his contributions to the development of Pakistan's nuclear technology and weapons program. He played a significant role in the country's nuclear tests conducted in May 1998, which made Pakistan the seventh nation in the world to possess nuclear weapons. Mubarakmand has also been involved in various scientific and technological initiatives in Pakistan and has held positions in academic and governmental institutions. His work primarily focuses on nuclear engineering and physics.

Pinned article: Introduction to the OurBigBook Project

Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
We have two killer features:
  1. topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculus
    Articles of different users are sorted by upvote within each article page. This feature is a bit like:
    • a Wikipedia where each user can have their own version of each article
    • a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
    This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
    Figure 1.
    Screenshot of the "Derivative" topic page
    . View it live at: ourbigbook.com/go/topic/derivative
  2. local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:
    This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
    Figure 2.
    You can publish local OurBigBook lightweight markup files to either https://OurBigBook.com or as a static website
    .
    Figure 3.
    Visual Studio Code extension installation
    .
    Figure 4.
    Visual Studio Code extension tree navigation
    .
    Figure 5.
    Web editor
    . You can also edit articles on the Web editor without installing anything locally.
    Video 3.
    Edit locally and publish demo
    . Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension.
    Video 4.
    OurBigBook Visual Studio Code extension editing and navigation demo
    . Source.
  3. https://raw.githubusercontent.com/ourbigbook/ourbigbook-media/master/feature/x/hilbert-space-arrow.png
  4. Infinitely deep tables of contents:
    Figure 6.
    Dynamic article tree with infinitely deep table of contents
    .
    Descendant pages can also show up as toplevel e.g.: ourbigbook.com/cirosantilli/chordate-subclade
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact