Some notable ones:
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it Filtration with vacuum pump by 
Ciro Santilli 40 Updated 2025-07-16
Ciro Santilli 40 Updated 2025-07-16The first thing we did was to filter the water samples with a membrane filter that is so fine that not even bacteria can pass through, but water can.
Therefore, after filtration, we would have all particles such as bacteria and larger dirt pieces in the filter.
From the 1 liter in each bottle, we only used 400 ml because previous experiments showed that filtering the remaining 600 ml is very time consuming because the membrane filter gets clogged up.
Therefore, the filtration step allows us to reduce those 400 ml volumes to more manageable Eppendorf tube volumes: Figure 1. "An Eppendorf tube". Reagents are expensive, and lab bench centrifuges are small!
Labelled Eppendorf tubes on a rack
. Source. Since the filter is so fine, filtering by gravity alone would take forever, and so we used a vacuum pump to speed thing up!
For that we used:
Peeling the vacuum pump filter protection peel before usage
. Source. 
Placing the vacuum pump filter
. Source. Programming languages without a decent dominating package system by Ciro Santilli 40 Updated 2025-07-16
Ciro Santilli 40 Updated 2025-07-16 Does the exact position of vertices matter in Feynman diagrams? by Ciro Santilli 40 Updated 2025-07-16
Ciro Santilli 40 Updated 2025-07-16Ciro Santilli once visited the chemistry department of a world leading university, and the chemists there were obsessed with NMR. They had small benchtop NMR machines. They had larger machines. They had a room full of huge machines. They had them in corridors and on desk tops. Chemists really love that stuff. More precisely, these are used for NMR spectroscopy, which helps identify what a sample is made of.
Introduction to NMR by Allery Chemistry
. Source. - only works with an odd number of nucleons
- apply strong magnetic field, this separates the energy of up and down spins. Most spins align with field.
- send radio waves into sample to make nucleons go to upper energy level. We can see that the energy difference is small since we are talking about radio waves, low frequency.
- when nucleon goes back down, it re-emits radio waves, and we detect that. TODO: how do we not get that confused with the input wave, which is presumably at the same frequency? It appears to send pulses, and then wait for the response.
How to Prepare and Run a NMR Sample by University of Bath (2017)
Source. This is a more direct howto, cool to see. Uses a Bruker Corporation 300. They have a robotic arm add-on. Shows spectrum on computer screen at the end. Shame no molecule identification after that!This video has the merit of showing real equipment usage, including sample preparation.
Says clearly that NMR is the most important way to identify organic compounds.
- youtu.be/uNM801B9Y84?t=41 lists some of the most common targets, including hydrogen and carbon-13
- youtu.be/uNM801B9Y84?t=124 ethanol example
- youtu.be/uNM801B9Y84?t=251 they use solvents where all protium is replaced by deuterium to not affect results. Genius.
- youtu.be/uNM801B9Y84?t=354 usually they do 16 radio wave pulses
NMR spectroscopy visualized by ScienceSketch
. Source. 2020. Decent explanation with animation. Could go into a bit more numbers, but OK. Why can't you collimate incoherent light as well as a laser? by Ciro Santilli 40 Updated 2025-07-16
Ciro Santilli 40 Updated 2025-07-16You could put an LED in a cavity with a thin long hole but then, most rays, which are not aligned with the hole, will just bounce inside forever producing heat.
So you would have a very hot device, and very little efficiency on the light output. This heat might also behave like a black-body radiation source, so you would not have a single frequency.
The beauty of lasers is the laser cavity (two parallel mirrors around the medium) selects parallel motion preferentially, see e.g.: youtu.be/_JOchLyNO_w?t=832 from Video "How Lasers Work by Scientized (2017)"
Just like computers, biological systems can be seen as being composed of several different layers of complexity.
Pinned article: Introduction to the OurBigBook Project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 3. Visual Studio Code extension installation.
Figure 4. Visual Studio Code extension tree navigation.
Figure 5. Web editor. You can also edit articles on the Web editor without installing anything locally.Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension.Video 4. OurBigBook Visual Studio Code extension editing and navigation demo. Source. 
- Infinitely deep tables of contents:
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact