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A component of the active eukaryotic CMG replicative helicase.
It works with MCM2-7 and GINS in the CMG helicase. Assembly and activation of this complex connect licensed replication origins to an operating fork; the presence of one component on chromatin does not alone establish active unwinding. [ref]
Molecular Biology of the Cell, 4th edition by Bruce Alberts and others. Chapters 4–7, chromatin, replication and gene regulation
Inappropriate recopying of already replicated nuclear DNA within a cell-cycle interval, prevented by controls separating origin licensing from firing.
Short RNA primers provide the free 3′ hydroxyl required by a DNA polymerase. Repeated priming is particularly important on the lagging strand, where synthesis proceeds through successive Okazaki fragments. [ref]
Molecular Biology of the Cell, 4th edition by Bruce Alberts and others. Chapters 4–7, chromatin, replication and gene regulation
Proliferating cell nuclear antigen, the eukaryotic sliding clamp that supports DNA synthesis and coordinates associated processes.
Replication factor C, a clamp-loading complex involved in installing PCNA on suitable DNA structures.
ATP-dependent clamp loading places the ring around a suitable primer-template junction. The clamp then supports a polymerase or associated factor, so loading the clamp and extending the DNA chain are different enzymatic operations. [ref]
Molecular Biology of the Cell, 4th edition by Bruce Alberts and others. Chapter 5, DNA Replication Mechanisms
A short DNA segment initiated on the lagging strand and later joined with neighbouring segments to produce a continuous daughter strand.
The E. coli replication-fork barrier involving Tus protein and Ter DNA sites, a specific bacterial termination arrangement rather than a universal bacterial mechanism.
The daughter strand synthesised predominantly in the direction of overall fork movement in conventional replication.
The daughter strand synthesised through repeated priming and joining of fragments because DNA polymerases extend only in one chemical direction.
Replication licensing prepares DNA replication origins for a subsequent initiation event. Separation of licensing from initiation and inhibition of relicensing during high-CDK phases helps prevent repeated copying within one cell cycle.
Impaired progression or completion of DNA replication. Stalled or damaged forks can lead to genome alterations if not appropriately stabilized, restarted or repaired.
Removal of a misincorporated terminal nucleotide by a suitable proofreading exonuclease, commonly acting in the 3′→5′ direction. It is distinct from later DNA mismatch repair.
Pinned article: Introduction to the OurBigBook Project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 2. You can publish local OurBigBook lightweight markup files to either OurBigBook.com or as a static website.
Figure 3. Visual Studio Code extension installation.
Figure 5. . You can also edit articles on the Web editor without installing anything locally. Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension. 
- Infinitely deep tables of contents:
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact