Cryogenic electron microscopy, which was developped in the 70's.
This could have been a Nobel Prize in Physics as well!
For the discovery of green fluorescent protein.
To Peter D. Mitchell for the discovery of the mechanism of ATP synthesis in the mitochondria, a central part of cellular respiration.
This was almost a two-in-one Nobel for a single dude: both for chemical bond theory and secondary structure theories such as the alpha helix! A beast.
Official page: www.nobelprize.org/prizes/chemistry/1954/summary
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