Cryogenic electron microscopy, which was developped in the 70's.
For the discovery of green fluorescent protein.
To Peter D. Mitchell for the discovery of the mechanism of ATP synthesis in the mitochondria, a central part of cellular respiration.
This was almost a two-in-one Nobel for a single dude: both for chemical bond theory and secondary structure theories such as the alpha helix! A beast.
Official page: www.nobelprize.org/prizes/chemistry/1954/summary
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