Arthur Ashkin's 1970 first experiment suspended transparent latex spheres in water inside a glass cell. A horizontal argon laser beam, focused to a radius, illuminated individual beads viewed through a microscope. An off-centre bead moved toward the beam axis and downstream until reaching the cell wall; blocking the beam released it into Brownian motion. Transparent particles and liquid reduced competing thermal effects. This observed sequence separates transverse attraction from downstream radiation pressure: wall-assisted confinement is not free three-dimensional confinement by one beam.[ref]
The beam-blocking comparison is particularly informative: persistence against a wall while illuminated and release when illumination stops test an optical cause. To infer a force from the downstream velocity, one additionally needs a linear drag model and the bead's distance from the wall; the observed motion alone does not calibrate an unrestricted harmonic optical trap.
One half went to Arthur Ashkin for optical tweezers and their biological applications. One quarter each went to Gérard Mourou and Donna Strickland for a method of generating intense, ultrashort optical pulses.[ref]
In 1986, Arthur Ashkin, Joseph Martin Dziedzic, John Ernst Bjorkholm and Steven Chu used spatially filtered argon laser light, a water-immersion objective of numerical aperture , and a water-filled glass cell. An auxiliary beam loaded large glass beads; dispersed small particles entered by Brownian motion. A side microscope and scattering of light detector provided readout. Their Fig. 1 shows a bead below the focus with about . On p. 290, silica size was inferred by scattering against a latex standard; translating the cell imposed fluid drag. These observations support single-beam confinement, while inferred size depends on the scattering model.[ref]
A loading beam can help bring a bead into the capture region without supplying its subsequent confinement. Distinguish loading, holding and sensing when interpreting an apparatus drawing. The following optical trap position readout is a proposed calibration arrangement; its detector layout is not a reconstruction of this historical experiment.