The common ones.
"De novo" means "starting from scratch", that is: you type the desired sequence into a computer, and the synthesize it.
The "de novo" part is important, because it distinguishes this from the already well solved problem of duplicating DNA from an existing DNA template, which is what all our cells do daily, and which can already be done very efficiently in vitro with polymerase chain reaction.
Notably, the dream of most of those companies is to have a machine that sits on a lab bench, which synthesises whatever you want.
The initial main applications are likely going to be:but the real pipe dream is building and bootstraping entire artificial chromosomes
- polymerase chain reaction primers (determine which region will be amplified
- creating a custom sequence to be inserted in a plasmid, i.e. artificial gene synthesis
News coverage:
- 2023-03 twitter.com/sethbannon/status/1633848116154880001
- 2020-10-05 www.nature.com/articles/s41587-020-0695-9 "Enzymatic DNA synthesis enters new phase"
Nuclera eDNA enzymatic de novo DNA synthesis explanatory animation (2021)
Source. The video shows nicely how Nuclera's enzymatic DNA synthesis works:- they provide blocked nucleotides of a single type
- add them with the enzyme. They use a werid DNA polymerase called terminal deoxynucleotidyl transferase that adds a base at a time to a single stranded DNA strand rather than copying from a template
- wash everything
- do deblocking reaction
- and then repeat until done
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it Qiagen DNeasy PowerWater Kit by 
Ciro Santilli 37 Updated 2025-05-13 +Created 1970-01-01
Ciro Santilli 37 Updated 2025-05-13 +Created 1970-01-01www.qiagen.com/gb/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powerwater-kit (archive) Here is its documentation: www.qiagen.com/gb/resources/download.aspx?id=bb731482-874b-4241-8cf4-c15054e3a4bf&lang=en (archive).
They put a lot of emphasis into base calling. E.g.:
- they have used FPGAs to accelerate it on certain models: twitter.com/nanopore/status/841671404588302338, sampe engineer: www.linkedin.com/in/balaji-renganathan-31b98415/
Quantum field theory lecture by Tobias Osborne (2017) Lecture 1 by Ciro Santilli 37 Updated 2025-05-13 +Created 1970-01-01
Ciro Santilli 37 Updated 2025-05-13 +Created 1970-01-01Bibliography review:
- Quantum Field Theory lecture notes by David Tong (2007) is the course basis
- quantum field theory in a nutshell by Anthony Zee (2010) is a good quick and dirty book to start
Course outline given:
- classical field theory
- quantum scalar field. Covers bosons, and is simpler to get intuition about.
- quantum Dirac field. Covers fermions
- interacting fields
- perturbation theory
- renormalization
Non-relativistic QFT is a limit of relativistic QFT, and can be used to describe for example condensed matter physics systems at very low temperature. But it is still very hard to make accurate measurements even in those experiments.
Mentions that "QFT is hard" because (a finite list follows???):But I guess that if you fully understand what that means precisely, QTF won't be too hard for you!
There are no nontrivial finite-dimensional unitary representations of the Poincaré group.
Notably, this is stark contrast with rotation symmetry groups (SO(3)) which appears in space rotations present in non-relativistic quantum mechanics.
www.youtube.com/watch?v=T58H6ofIOpE&t=5097 describes the relativistic particle in a box thought experiment with shrinking walls
Pinned article: ourbigbook/introduction-to-the-ourbigbook-project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 2. You can publish local OurBigBook lightweight markup files to either OurBigBook.com or as a static website.
Figure 3. Visual Studio Code extension installation.
Figure 5. . You can also edit articles on the Web editor without installing anything locally. Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension. 
- Infinitely deep tables of contents:
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact