Ciro Santilli @cirosantilli 37

Our definition is: about which culture a film is. Not who paid for it.

A non-tool-assisted speedrun.

Ciro Santilli views humans as biological robots, and therefore RTA videos can be thought of as probabilistic TAS with human achievable reflex constraints.

This aspect is especially highlighted in "speed run record evolution videos", which can be quite fun, e.g. www.youtube.com/watch?v=pmS9e7kzgS4 Ocarina of Time - World Record History and Progression (Any% Speedrun, 1990s-2017) by retro (2017)

From a similar point of view, Ciro also sometimes watches/learns a bit about competitive PvP games from a "could a computer play this better than a human" point of view.

Ciro also likes to watch commented manual speedruns of games as a way of experiencing the game at a high level without spending too much time on it, often from Games Done Quick. Their format is good because it generally showcases one player focusing more on the gameplay, and three couch commentators to give context, that's a good setup.

It is a

A directed weighted graph where the sum of weights of all outgoing edges equals 1.

A bit over the top, but convincing enough. A crypto-vampire show.

A member of a tangent space.

Also known as: Quantitative PCR (qPCR).

Like PCR, but the amplification machine measures the concentration of DNA at each step.

This describes one possible concentration detection method with fluorescent molecules that only become fluorescent when the DNA is double stranded (SYBR Green)

This allows you to predict the exact initial concentration by extrapolating the exponential curve backwards.

TODO: vs non-real-time PCR. Why can't you just divide by 2 for every heating step to reach back the original concentration? Likely the reaction reach saturation at an unknown step.

TODO: vs non-real-time PCR in medical diagnostics: do you really need to know concentration for diagnostics? Isn't it enough to know if the virus is present or not?