Interferometer by Ciro Santilli 34 Updated +Created
Polarizer by Ciro Santilli 34 Updated +Created
Sequelize by Ciro Santilli 34 Updated +Created
Some usage examples under: Section "Sequelize example".
As of 2021, this library is extremely painful to use. It does feel semi-mature, but there are just too much horrible things going on;
Some glaring issues are listed at the horrors of Sequelize.
Adenine nucleotide translocator by Ciro Santilli 34 Updated +Created
A single transmembrane protein that moves ATP out and ADP in of the mitochondrion. So crucial.
Present in chormosome 4.
Video 1.
Energized about the Mechanism of ADP/ATP Transport by Ruprecht et al. (2019)
Source.
Good video showing what appears to be the adenine nucleotide translocator. although they don't use that name, instead saying ADP/ATP carrier.
The video also briefly depicts the ATP synthase and the mitochondrial phosphate carrier protein.
Serial wire debug by Ciro Santilli 34 Updated +Created
Non-ionizing radiation by Ciro Santilli 34 Updated +Created
Sycamore processor by Ciro Santilli 34 Updated +Created
This is a good read: quantumai.google/hardware/datasheet/weber.pdf May 14, 2021. Their topology is so weird, not just a rectangle, one wonders why! You get different error rates in different qubits, it's mad.
Figure 1.
Google Sycamore Weber quantum computer connectivity graph
. Weber is a specific processor of the Sycamore family. From this we see it clearly that qubits are connected to at most 4 other qubits, and that the full topology is not just a simple rectangle.
Tachyonic antitelephone by Ciro Santilli 34 Updated +Created
.rel.text by Ciro Santilli 34 Updated +Created
Besides sh_type == SHT_RELA, there also exists SHT_REL, which would have section name .text.rel (not present in this object file).
Those represent the same struct, but without the addend, e.g.:
typedef struct {
    Elf64_Addr  r_offset;
    Elf64_Xword r_info;
} Elf64_Rela;
The ELF standard says that in many cases the both can be used, and it is just a matter of convenience.
Continent by Ciro Santilli 34 Updated +Created
Time series run variant by Ciro Santilli 34 Updated +Created
To modify the nutrients as a function of time, with To select a time series we can use something like:
python runscripts/manual/runSim.py --variant nutrientTimeSeries 25 25
As mentioned in python runscripts/manual/runSim.py --help, nutrientTimeSeries is one of the choices from github.com/CovertLab/WholeCellEcoliRelease/blob/7e4cc9e57de76752df0f4e32eca95fb653ea64e4/models/ecoli/sim/variants/__init__.py#L57
25 25 means to start from index 25 and also end at 25, so running just one simulation. 25 27 would run 25 then 26 and then 27 for example.
The timeseries with index 25 is reconstruction/ecoli/flat/condition/timeseries/000025_cut_aa.tsv and contains
"time (units.s)" "nutrients"
0 "minimal_plus_amino_acids"
1200 "minimal"
so we understand that it starts with extra amino acids in the medium, which benefit the cell, and half way through those are removed at time 1200s = 20 minutes. We would therefore expect the cell to start expressing amino acid production genes exactly at that point.
nutrients likely means condition in that file however, see bug report with 1 1 failing: github.com/CovertLab/WholeCellEcoliRelease/issues/24
When we do this the simulation ends in:
Simulation finished:
 - Length: 0:34:23
 - Runtime: 0:08:03
so we see that the doubling time was faster than the one with minimal conditions of 0:42:49, which makes sense, since during the first 20 minutes the cell had extra amino acid nutrients at its disposal.
The output directory now contains simulation output data under out/manual/nutrientTimeSeries_000025/. Let's run analysis and plots for that:
python runscripts/manual/analysisVariant.py &&
python runscripts/manual/analysisCohort.py --variant 25 &&
python runscripts/manual/analysisMultigen.py --variant 25 &&
python runscripts/manual/analysisSingle.py --variant 25
We can now compare the outputs of this run to the default wildtype_000000 run from Section "Install and first run".
  • out/manual/plotOut/svg_plots/massFractionSummary.svg: because we now have two variants in the same out/ folder, wildtype_000000 and nutrientTimeSeries_000025, we now see a side by side comparision of both on the same graph!
    The run variant where we started with amino acids initially grows faster as expected, because the cell didn't have to make it's own amino acids, so growth is a bit more efficient.
    Then, at 20 minutes, which is about 0.3 hours, we see that the cell starts growing a bit less fast as the slope of the curve decreases a bit, because we removed that free amino acid supply.
    Figure 1.
    Minimal condition vs amino acid cut mass fraction plot
    . Source. From file out/manual/plotOut/svg_plots/massFractionSummary.svg.
The following plots from under out/manual/wildtype_000000/000000/{generation_000000,nutrientTimeSeries_000025}/000000/plotOut/svg_plots have been manually joined side-by-side with:
for f in out/manual/wildtype_000000/000000/generation_000000/000000/plotOut/svg_plots/*; do
  echo $f
  svg_stack.py \
    --direction h \
    out/manual/wildtype_000000/000000/generation_000000/000000/plotOut/svg_plots/$(basename $f) \
    out/manual/nutrientTimeSeries_000025/000000/generation_000000/000000/plotOut/svg_plots/$(basename $f) \
    > tmp/$(basename $f)
done
Figure 2.
Amino acid counts
. Source. aaCounts.svg:
  • default: quantities just increase
  • amino acid cut: there is an abrupt fall at 20 minutes when we cut off external supply, presumably because it takes some time for the cell to start producing its own
Figure 3.
External exchange fluxes of amino acids
. Source. aaExchangeFluxes.svg:
  • default: no exchanges
  • amino acid cut: for all graphs except phenylalanine (PHE), either the cell was intaking the AA (negative flux), and that intake goes to 0 when the supply is cut, or the flux is always 0.
    For PHE however, the flux is at all times, except shortly after the cut. Why? And why there was no excretion on the default conditions?
Figure 4.
Evaluation time
. Source. evaluationTime.svg: this has nothing to do with biology, but it is rather a profile of the program runtime. We can see that the simulation gets slower and slower as time passes, presumably because there are more and more molecules to simulate.
Figure 5.
mRNA count of highly expressed mRNAs
. Source. From file expression_rna_03_high.svg. Each of the entries is a gene using the conventional gene naming convention of xyzW, e.g. here's the BioCyc for the first entry, tufA: biocyc.org/gene?orgid=ECOLI&id=EG11036, which comments
Elongation factor Tu (EF-Tu) is the most abundant protein in E. coli.
and
In E. coli, EF-Tu is encoded by two genes, tufA and tufB
. What they seem to mean is that tufA and tufB are two similar molecules, either of which can make up the EF-Tu of the E. Coli, which is an important part of translation.
Figure 6.
External exchange fluxes
. Source.
mediaExcange.svg: this one is similar to aaExchangeFluxes.svg, but it also tracks other substances. The color version makes it easier to squeeze more substances in a given space, but you lose the shape of curves a bit. The title seems reversed: red must be excretion, since that's where glucose (GLC) is.
The substances are different between the default and amino acid cut graphs, they seem to be the most exchanged substances. On the amino cut graph, first we see the cell intaking most (except phenylalanine, which is excreted for some reason). When we cut amino acids, the uptake of course stops.
E. Coli K-12 MG1655 promoter by Ciro Santilli 34 Updated +Created
From this we see that there is a convention of naming promoters as protein name + p, e.g. the first gene in E. Coli K-12 MG1655 promoter thrLp encodes protein thrL.
It is also possible to add numbers after the p, e.g. at biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=PM0-45989 we see that the protein zur has two promoters:
  • zurp6
  • zurp7
TODO why 6 and 7? There don't appear to be 1, 2, etc.
ILoveYouMore.jpg by Ciro Santilli 34 Updated +Created
This is the first of many love declarations and mentions EMBII makes of his partner Chiharu! This came just one day afte the very first uploads of the system.
Figure 1.
ILoveYouMore.jpg
. Source.
Message:
My Dearest Chiharu....I Love you more. <3 Eric
Note that she's Japanese and not really bond, it's hair dye.
Figure 2.
OurWedding.jpg
. Source.
Message:
My Dearest Chiharu, I will love you forever. Taken Aug 6th 2014 in Ipswich, SD.
Figure 3.
MadyBobbyOffToCollege.jpg
. Source.
Associated messages:
A #father could not ask for more perfect daughters. I #Love you both so much!! <3 Pa
My oldest daugher moves into the dorms tomorrow morning. Dear Mady, You are forever my baby. <3 PaEMBII's daughter, Maddy Bobby, (presumably not with Chiharu) is going off to college! Sadface.
Figure 4.
Chiharu EMBII and The Atom Sea say Happy Halloween.jpg
. Source.
Message:
#Chiharu #embii & the #AtomSea #Fargo #ND
so their location was: en.wikipedia.org/wiki/Fargo,_North_Dakota
Figure 5.
Chiharu.jpg
. Source.
Messages:
mini camera test #Wilson #Chiharu #embii #Broadway #Fargo #ND
and:
"Trip to Italy" Mini Digital Camera N. Broadway, Fargo, ND 49°F
TODO actual Italy? Or some place named Italy in the US?
Figure 6.
Loraine.jpg
. Source.
Photographer unknown, but presumably EMBII's father or another close family member.
Message:
In loving memory of Loraine Elizabeth WhiteEMBII's mum died :-(
Cost: ~0.001 BTC ~ $0.80 at the time.
Figure 7.
SatoFamily.jpg
. Source.
This one gives Chiharu's full identity with picture basically. Message:
The Sato Family Arrives from Japan! Taken Aug 2. 2014 in Minneapolis MN. (Keiko, Chiharu, Hideaki, Katsuhiko) Now preparing for the Sato / Bobby Great American Vacation!!
so presumably Chiharu's full name is Chiharu Sato.
More EMBII social media:
Ionic bond by Ciro Santilli 34 Updated +Created
OpenWorm by Ciro Santilli 34 Updated +Created
High level simulation only, no way to get from DNA to worm! :-) Includes:
3D body viewer at: browser.openworm.org/ TODO can you click on a cell to get its name?
Video 1.
OpenWorm Sibernetic demo by Mike Vella (2013)
Source. Sibernetic adds a fluid dynamics solver for brain-in-the-loop simulation of C. elegans.
f-orbital by Ciro Santilli 34 Updated +Created
Fermentation by Ciro Santilli 34 Updated +Created
TLB replacement policy by Ciro Santilli 34 Updated +Created
When TLB is filled up, older addresses are overwritten. Just like CPU cache, the replacement policy is a potentially complex operation, but a simple and reasonable heuristic is to remove the least recently used entry (LRU).
With LRU, starting from state:
  valid  linear  physical
  -----  ------  --------
> 1      00003   00005
  1      00007   00009
  1      00009   00001
  1      0000B   00003
adding 0000D -> 0000A would give:
  valid  linear  physical
  -----  ------  --------
  1      0000D   0000A
> 1      00007   00009
  1      00009   00001
  1      0000B   00003

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