Contains the genes: e. Coli K-12 MG1655 gene thrL, e. Coli K-12 MG1655 gene thrA, e. Coli K-12 MG1655 gene thrB and e. Coli K-12 MG1655 gene thrC, all of which have directly linked functionality.
We can find it by searching for the species in the BioCyc promoter database. This leads to: biocyc.org/group?id=:ALL-PROMOTERS&orgid=ECOLI.
By finding the first operon by position we reach: biocyc.org/ECOLI/NEW-IMAGE?object=TU0-42486.
That page lists several components of the promoter, which we should try to understand!
Some of the transcription factors are proteins:
After the first gene in the codon, thrL, there is a rho-independent termination. By comparing:we understand that the presence of threonine or isoleucine variants, L-threonyl and L-isoleucyl, makes the rho-independent termination become more efficient, so the control loop is quite direct! Not sure why it cares about isoleucine as well though.
TODO which factor is actually specific to that DNA region?
www.facebook.com/watch/live/?mibextid=qC1gEa&ref=watch_permalink&v=10100156534675351 claims to have a video of Benji Wojin and his cousin Marco Troper from 2018. www.linkedin.com/posts/superintendents_marco-and-benji-wojin-love-these-kids-and-activity-7164448664772435968-siUC/ that links to it is lamenting Marco Troper 's death in 2024.
Claimed photos can be found at:All of the above are likely of the same child.
sigoden/node-express-realworld-example-app by 
Ciro Santilli 35 Updated 2025-04-05 +Created 1970-01-01
Ciro Santilli 35 Updated 2025-04-05 +Created 1970-01-01Appears to be a port of gothinkster/node-express-realworld-example-app to Sequelize.
Seemed to just work at 68bbadfd77f679f0df0fcd0de5bceb9c37b1144a Ubuntu 20.10, was forked from parent project in 2018.
- www.raspberrypi.com/documentation/microcontrollers/c_sdk.html
- github.com/raspberrypi/pico-sdk
- github.com/raspberrypi/pico-examples The key hello world examples are:
Ubuntu 22.04 build just worked, nice! Much feels much cleaner than the Micro Bit C setup:
sudo apt install cmake gcc-arm-none-eabi libnewlib-arm-none-eabi libstdc++-arm-none-eabi-newlib
git clone https://github.com/raspberrypi/pico-sdk
cd pico-sdk
git checkout 2e6142b15b8a75c1227dd3edbe839193b2bf9041
cd ..
git clone https://github.com/raspberrypi/pico-examples
cd pico-examples
git checkout a7ad17156bf60842ee55c8f86cd39e9cd7427c1d
cd ..
export PICO_SDK_PATH="$(pwd)/pico-sdk"
cd pico-exampes
mkdir build
cd build
# Board selection.
# https://www.raspberrypi.com/documentation/microcontrollers/c_sdk.html also says you can give wifi ID and password here for W.
cmake -DPICO_BOARD=pico_w ..
make -jThen we install the programs just like any other UF2 but plugging it in with BOOTSEL pressed and copying the UF2 over, e.g.:Note that there is a separate example for the W and non W LED, for non-W it is:
cp pico_w/blink/picow_blink.uf2 /media/$USER/RPI-RP2/cp blink/blink.uf2 /media/$USER/RPI-RP2/Also tested the UART over USB example:You can then see the UART messages with:
cp hello_world/usb/hello_usb.uf2 /media/$USER/RPI-RP2/screen /dev/ttyACM0 115200TODO understand the proper debug setup, and a flash setup that doesn't require us to plug out and replug the thing every two seconds. www.electronicshub.org/programming-raspberry-pi-pico-with-swd/ appears to describe it, with SWD to do both debug and flash. To do it, you seem need another board with GPIO, e.g. a Raspberry Pi, the laptop alone is not enough.
Matches the quantum superposition probability proportional to the square law. Poor Étienne-Louis Malus, who died so much before this was found.
The first thing we had to do with the sample was to extract the DNA present in the water in a pure form for the PCR.
We did that with a Qiagen DNeasy PowerWater Kit.
As you would expect, this consists of a purification procedure with several steps.
In each step we take a physical or chemical action on the sample, which splits it into two parts: the one with the DNA and the one without.
We then take the part with the DNA, and throw away the one without the DNA.
The first steps are coarser, and finer and finer splits are done as we move forward.
How Microwaves Work by National MagLab (2017)
Source. A bit meh. Does not mention the word cavity magnetron!How a Microwave Oven Works by EngineerGuy
. Source. Cool demonstration of the standing waves in the cavity with cheese! Essential metabolism for a minimal cell (2019) by Ciro Santilli 35 Updated 2025-04-05 +Created 1970-01-01
Ciro Santilli 35 Updated 2025-04-05 +Created 1970-01-01Seems to be a GNU Binutils extension
The fifth gene, and the first E. Coli K-12 MG1655 gene of unknown function as of 2021.
Chemistry course of the University of Oxford by Ciro Santilli 35 Updated 2025-04-05 +Created 1970-01-01
Ciro Santilli 35 Updated 2025-04-05 +Created 1970-01-01Handbook 2019/2020: web.archive.org/web/20210211192812/http://teaching.chem.ox.ac.uk/Data/Sites/58/media/courseinfo/ug-handbook-chemistry-2019-20.pdf
At teaching.chem.ox.ac.uk/undergraduate-course-handbook.aspx there's a paywall, but Google found the PDF it anyways.
www.ox.ac.uk/students/academic/guidance/undergraduate/handbooks in theory links to all handbooks, but some are likely paywalled. But Google can generally find them anyways.
There are unlisted articles, also show them or only show them.