Ciro Santilli @cirosantilli 37

France is obviously a fine rich place to live, Ciro Santilli lived there for a few years.

The first/only way Ciro could find was with ampy: stackoverflow.com/questions/74150782/how-to-run-a-micropython-host-script-file-on-the-raspbery-pi-pico-from-the-host/74150783#74150783 That just worked and it worked perfectly!

TODO: possible with rshell?

An upstream repo at: github.com/raspberrypi/pico-micropython-examples

Some generic Micropython examples most of which work on this board can be found at: Section "MicroPython example".

Pico W specific examples are under: rpi-pico-w/upython.

The examples can be run as described at Program Raspberry Pi Pico W with MicroPython.

www.qiagen.com/gb/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powerwater-kit (archive) Here is its documentation: www.qiagen.com/gb/resources/download.aspx?id=bb731482-874b-4241-8cf4-c15054e3a4bf&lang=en (archive).

Manual archive: web.archive.org/web/20190911111136/https://www.qiagen.com/gb/resources/download.aspx?id=bb731482-874b-4241-8cf4-c15054e3a4bf&lang=en

Kit to extract clean DNA from water.

At the time of the experiment, Illumina equipment was cheaper per base pair and dominates the human genome sequencing market, but it required a much higher initial investment for the equipment (TODO how much).

The reusable Nanopore device costs just about 500 dollars, and about 500 dollars (50 unit volume) for the single usage flow cell which can decode up to 30 billion base pairs, which is about 10 human genomes 1x! Note that 1x is basically useless for one of the most important of all applications of sequencing: detection of single-nucleotide polymorphisms, since the error rate would be too high to base clinical decisions on.

Compare that to Illumina which is currently doing about an 1000 dollar human genome at 30x, and a bit less errors per base pair (TODO how much).

Other advantages of the MinION over Illumina which didn't really matter to this particular experiment are:

www.gelifesciences.com/en/us/shop/protein-analysis/protein-sample-preparation/protein-enrichment/magrack-6-p-05761 (archive).

For the most part, a great pseudo-country to live in with lots of cultural diversity, art and safety.

However, Europe is in economic decline after all its Jewish and German geniuses fled in/after World War II and due to having more than one natural language is bad for the world.

Ciro Santilli moved to Europe in 2010.

Higher spin multiplicity means lower energy. I.e.: you want to keep all spins pointin in the same direction.

Contains the genes: e. Coli K-12 MG1655 gene thrL, e. Coli K-12 MG1655 gene thrA, e. Coli K-12 MG1655 gene thrB and e. Coli K-12 MG1655 gene thrC.

Happening multiple times a day on Ubuntu 22.04 for Chromium, even though I turn computer on and off daily, unbearable: