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An inclusion body in recombinant protein production is an insoluble aggregate-rich fraction containing the expressed material. Abundance does not demonstrate native folding; any recovered refolded product needs independent identity, assembly and activity checks.
Folding while a chain is being synthesized. Changes in elongation timing can affect the resulting folded and functional fraction.
Rapid removal of an existing protein pool to separate immediate effects from later adaptation. Depletion kinetics and perturbation specificity need direct measurement.
Use of ester protecting groups that can be selectively removed under different conditions. A methyl/tert-butyl pair can permit kinetic base hydrolysis at one end and acid-promoted alkyl cleavage at the other.
A cyclic acetal protecting group made from an alcohol and dihydropyran. It protects the original alcohol oxygen and can be removed under suitable acidic hydrolysis conditions.
A progenitor cell with competence for neural and mesodermal development, particularly spinal cord and paraxial mesoderm. Shared markers indicate cell state; demonstrating realised bipotency requires clonal lineage tracing.
One of the early germline precursor cells formed at the embryonic poles in Drosophila melanogaster, normally at the posterior where germ plasm is assembled.
An experimentally induced cell resembling an embryonic primordial germ cell by molecular and developmental criteria. Similarity should be stated separately from demonstrated gamete-forming function.
Random variation represented by a probability distribution or stochastic process, whether from intrinsic events or an uncertain environment.
The rate of consumption by an individual consumer as a function of resource abundance under specified conditions. Encounter, handling and satiation can generate different forms.
Incorporation or removal of an otherwise soluble component during precipitation of another material. Surface adsorption, occlusion of solution and incorporation in the growing solid can make precipitation less selective than a solubility-product calculation alone suggests.
The fraction of molecules modified at a specified site under specified conditions. Occupancies at separate sites do not alone establish the joint distribution of modifications across intact proteoforms.
Pinned article: Introduction to the OurBigBook Project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 2. You can publish local OurBigBook lightweight markup files to either OurBigBook.com or as a static website.
Figure 3. Visual Studio Code extension installation.
Figure 5. . You can also edit articles on the Web editor without installing anything locally. Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension. 
- Infinitely deep tables of contents:
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact