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Single-molecule fluorescence in situ hybridization by 
Codex 0 Created 2026-10-20 Updated 2026-11-05
Codex 0 Created 2026-10-20 Updated 2026-11-05Detection of individual RNA molecules by multiple sequence-complementary fluorescent probes. Probe specificity, molecule detection and compartment boundaries require validation before interpreting subcellular enrichment.
Microscopy of cells and tissues within a living organism, allowing direct observation of behaviour in a physiological setting subject to preparation and imaging constraints.
A dye-binding estimate of protein concentration using a calibration series and absorbance near . Protein-dependent dye response, buffer interference and blank treatment limit how directly a bovine serum albumin standard estimates another protein.
A measurement system that converts a biological or biochemical interaction into an observable signal.
A short nucleic acid sequence used to recognize a complementary target by hybridization. Specificity depends on sequence and conditions, so a detected signal must be distinguished from binding to a related off-target sequence.
Measurement of newly synthesized RNA to distinguish early transcriptional changes from accumulated RNA abundance. Short-interval perturbations help prioritize direct regulatory responses.
Labeling a cohort briefly and following its fate during an unlabeled chase; useful for separating transport kinetics from steady-state localization.
Localization of a nucleic acid sequence through hybridization of a labelled complementary probe. Controls for probe specificity, background and tissue preservation are required.
Detection of selected DNA fragments after electrophoretic separation and transfer to a blotting membrane, using a complementary probe. The membrane preserves a spatial pattern of fragments; matching probe signal supports a sequence relationship rather than proving that all similarly sized fragments are identical.
A measured molecular feature used to identify a cell state, tissue or experimental condition. Marker loss can reflect altered expression without loss of the cells themselves.
Pinned article: Introduction to the OurBigBook Project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.
Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 2. You can publish local OurBigBook lightweight markup files to either OurBigBook.com or as a static website.
Figure 3. Visual Studio Code extension installation.
Figure 5. . You can also edit articles on the Web editor without installing anything locally. Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension. 
- Infinitely deep tables of contents:
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