The optical pupil mapped onto the deformable mirror has actuator pitch approximately . A sinusoidal wavefront error must have at least two actuator intervals per period to avoid aliasing, by the Nyquist–Shannon sampling theorem. Thus
If the actuator centres include both endpoints of the optical pupil diameter, the literal pitch is and the corresponding expression is ; the paper uses the customary large- pitch convention. At the exact Nyquist boundary one quadrature can be poorly sampled, and real influence functions and actuator coupling reduce useful correction near the cutoff. The result is an ideal sampling bound, not a guarantee of arbitrary correction at its endpoint.
An optical pupil ripple of spatial period diffracts starlight into two sidebands displaced by along the ripple's spatial frequency vector. Substituting the axial Nyquist period gives the deformable-mirror control radius
The square actuator lattice has component cutoffs , hence an ideal square corrected region with this half-width in each photodetector direction. Its diagonal reaches times the axial radius; an isotropic conservative restriction is the inscribed disk. Using as the scale of a spatial resolution element, the square is about elements on a side and in area; the disk has about elements. These are angular area counts, not the quadrant coefficient count in part (ii).
A circular illuminated optical pupil has fewer active actuator degrees of freedom than the complete square array, and influence functions alter the usable boundary. Moreover one real wave phase deformable mirror produces conjugately related corrections at opposite speckles: arbitrary complex-field correction of both independent wave amplitude and wave phase errors generally needs additional control, or a restricted half-plane. The square therefore describes ideal spatial-frequency access, rather than a guarantee that every intensity element inside it can be independently set to zero. The Fourier-domain square control region is derived in Bordé and Traub's speckle-nulling analysis.
A finite optical pupil spreads a point into a point spread function. In incoherent imaging an extended intensity distribution is convolved with that function; its Fourier transform is the optical transfer function, which attenuates and ultimately removes high spatial frequencies. For an ideal circular pupil the point response is an Airy disk. The Rayleigh criterion for two equally bright incoherent points is
A microscope's object-space scale is of order , with the numerical factor depending on illumination and the criterion; measures the accepted ray cone. A larger pupil or shorter wavelength improves the diffraction scale. Aberrations, defocus, detector pixel sampling, motion, background and photon noise can worsen practical performance. Merely magnifying the same blurred image adds no information. Coherent sources require amplitude superposition instead of the incoherent intensity rule. Rayleigh resolution is a convention for separating a pair, distinct from the precision of localizing one isolated point; information does not abruptly vanish below it.
Phase-contrast microscopy Created 2026-10-14 Updated 2026-10-18
Phase-contrast microscopy converts specimen-dependent phase differences into visible light intensity differences. It can reveal structures in living, unstained biological cells, allowing their changes to be followed without first making an absorbing preparation.[ref] The key question is how an optical microscope can read a phase when an ordinary detector records light intensity.
Consider a transparent specimen with complex amplitude transmission . In ideal in-focus coherent imaging with all relevant spatial frequencies transmitted, its image has : varying alone produces no brightness detail. This is a particular model, not a claim that all transparent specimens are invisible. Diffraction, defocus, absorption and a finite optical pupil can already change a real image.
A phase-contrast microscope images an illuminating annulus onto a phase plate ring near the objective's rear focal plane. The aligned ring attenuates and shifts direct light relative to most diffracted light; their interference changes image brightness.[ref]
Figure 1.
Separating the reference light before recombining the image
. Original functional diagram of phase-contrast microscopy. The annulus and ring indicate their optical correspondence, not an exact ray prescription. The lower complex amplitude construction uses the original weak phase object model below, with chosen positive specimen phase and two opposite quarter-cycle reference shifts. Details.
The method is beautiful because the bright direct beam becomes its own reference: a small specimen-dependent complex amplitude can interfere with it and change the image at first order. Merely magnifying an invisible phase pattern would leave unchanged.
Rayleigh criterion 2026-10-14
The Rayleigh criterion calls two equal, mutually incoherent optical point sources just resolved when one circular-pupil Airy disk's centre coincides with the other's first zero. It is a specified discrimination convention, distinct from full width at half maximum and from precision in estimating one source's position.
For this circular optical pupil, the Rayleigh criterion uses angular separation , or in the paraxial approximation to the focal plane. The point spread functions still overlap.[ref]
For the Airy disk design example with , and , the angular convention gives , or about arcseconds, and the image-plane separation is . Two equally bright, mutually incoherent optical point sources at separations , and times this scale would have image separations , and respectively. These calculated cases are compared in incoherent two-source Airy profile.
The Rayleigh criterion is useful because it ties an easily recognized two-peak convention to a predictable optical scale. It does not assert that all information vanishes below that separation. Inferring two unknown positions also depends on photon shot noise, background, unequal brightness and uncertainty in the point spread function; locating a single isolated source is a different task. A theoretical analysis shows that alternative optical measurements can estimate the separation of two incoherent optical point sources below this scale.[ref]
The mutual optical coherence condition is essential. In incoherent imaging, time-averaged light intensities add. In coherent imaging, wave amplitudes add before squaring, leaving a wave interference term controlled by relative wave phase. Two holes illuminated by the same coherent laser therefore do not implement the equal-incoherent-source convention merely because their individual patterns resemble Airy disks. The coherent single-aperture laboratory test establishes the response of each aperture; it does not establish the pair's mutual incoherence.
Seidel coma pupil mapping 2026-10-14
Consider an original, explicitly normalized model of primary coma, rather than a prescription for a particular optical lens. Let be Cartesian coordinates in a circular optical pupil divided by its radius , with . Let be a signed small field angle divided by an arbitrary positive reference angle. With polar coordinates , , prescribe the wavefront error
Here is an arbitrary positive optical path difference scale, not a measured error. The cubic optical pupil dependence and linear field dependence are the classical Seidel form.[ref]
In the paraxial approximation, light ray direction errors follow the transverse gradient of wavefront error. For a reference sphere a distance from the exit optical pupil in a uniform image medium of refractive index , the corresponding image-error scale is . Choose image-error coordinate signs so the normalized displacement is ; reversing the convention reverses the plotted orientation. Image coordinates below are errors relative to the ideal image of this particular object point, rather than positions of different objects:
The partial derivatives explain the asymmetry: opposite optical pupil points and land together, rather than on opposite sides of the ideal image. For , every is nonnegative, while can have either sign. The figures stipulate this single wavefront error, omit other optical aberrations and diffraction, and assign no instrument dimensions.
For a ring of fixed optical pupil radius , the double-angle formulas for trigonometric functions give
Thus each ring maps into a circle of radius whose centre is displaced by . Doubling quadruples both sizes; for , the edge ring has centre and radius , while the half-radius ring has centre and radius . Superposing these displaced circles produces the comet-like envelope.
Figure 1.
Primary coma: optical pupil rings map into displaced image circles
. Original normalized Seidel coma pupil mapping at . Colours identify equal-radius optical pupil rings and their image circles; crosses mark circle centres. The opposite points A and B have the same image position. These are light ray intersections, with no instrument dimensions or diffraction calculation. Details.
For uniform irradiance over the circular optical pupil, equal area samples give an equal-weight geometrical image. Its centroid is because and . Moving the coordinate origin to that centroid translates the image; it does not remove its asymmetric shape. Adding a linear term to likewise translates all light rays equally. These origin choices must be distinguished from changing the optical aberration itself.
Figure 2.
The normalized geometrical point image grows and reverses with field angle
. Original equal-area sampling of the same circular optical pupil, mapped at with one image scale. The orange cross is the geometrical centroid. Each blue point represents one equal-weight light ray; point density is not a calibrated light intensity display or a measured point spread function. At all model light rays coincide, while a real finite aperture still gives diffraction. Details.
The model's full transverse extent is along and along . Halving the field angle halves the blur, and reversing it reverses the pattern about the ideal image. This isolates why coma matters for an optical camera's usable field of view: collecting more light does not ensure that off-axis points remain compact. Near diffraction-limited resolution, a physical optics calculation of the point spread function is needed instead of interpreting the geometrical tip as an infinitely sharp image.[ref]