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Pasteurella multocida is a species of Gram-negative bacteria associated with animal hosts and diseases, including fowl cholera. Different bacterial strains can differ in host association and molecular properties; one sequenced isolate does not exhaust the diversity of the species.[ref]
Complete genomic sequence of Pasteurella multocida, Pm70 by Barbara J. May and others. Abstract and Introduction: organism and avian Pm70 isolate
In 2001, investigators reported the genome of the avian isolate Pm70, predicting 2,014 coding sequences. Sequence comparisons identified candidate functions associated with virulence and iron acquisition. This supplied testable molecular leads, rather than direct proof that every predicted gene caused disease.[ref]
Complete genomic sequence of Pasteurella multocida, Pm70 by Barbara J. May and others. Abstract and Table1: coding sequences and candidate functions
An existing biochemical example makes the distinction between prediction and measurement concrete. Work on its hyaluronan synthase characterized use of two activated sugar substrates to build hyaluronic acid, whose chain alternates glucuronic acid and N-acetylglucosamine. An enzyme assay tests the actual chemical activity of a preparation; a sequence match proposes a possible activity that still needs such tests.[ref]
Enzymological characterization of the Pasteurella multocida hyaluronic acid synthase by P. L. DeAngelis. Abstract: substrates and enzymological characterization
The species name, bacterial strain, genome annotation and measured enzyme function therefore answer different biological questions. Keeping them distinct makes comparisons among isolates more informative than assigning every property of one isolate to all members.
A bacterial strain is a maintained lineage of bacteria descended from a particular isolation in pure culture. It is more specific than a species name. The naming code describes descent from an isolation and subsequent cultures; it does not require every cell forever to retain an identical genome.[ref]
International Code of Nomenclature of Prokaryotes. Prokaryotic Code (2022 Revision) by Aharon Oren and others. Appendix 10, A(3), "Use of other terms": definition of a strain and succession of cultures
A concrete example is Pm70, the avian isolate used for the published Pasteurella multocida genome. Naming both the species and the strain identifies the particular material behind a sequence or assay. The 2001 genome paper reported 2,014 predicted coding sequences for Pm70, not a measurement of every member of the species.[ref]
Complete genomic sequence of Pasteurella multocida, Pm70 by Barbara J. May and others. Abstract, Introduction and Table1: Pm70 isolate and genome annotation
For an illustrative experiment, compare a parent strain with a derivative in which one candidate gene has been removed, keeping culture conditions comparable. A change in the measured phenotype tests a claim about that derivative under those conditions. It does not make the two cultures different species, nor establish that all other strains would respond identically.
Sphingomonas is a taxonomic genus of bacteria. Its type species is Sphingomonas paucimobilis; the genus name is validly published in the prokaryotic naming system. A bacterial strain, an individual species and the genus are different levels of identification.[ref]
A2011 experiment made that distinction consequential. Researchers grew Arabidopsis thaliana with or without candidate bacterial colonists, then challenged the plants with the leaf pathogen Pseudomonas syringae. Many plant-derived Sphingomonas isolates reduced both disease symptoms and pathogen growth. Isolates obtained from air, dust or water did not necessarily protect, even at comparable bacterial densities on the plant.[ref]
Protection of Arabidopsis thaliana against leaf-pathogenic Pseudomonas syringae by Sphingomonas strains in a controlled model system by Gerd Innerebner and others. Abstract, controlled challenge and isolate-source comparison
The experiment distinguishes colonization from a protective function. Finding a Sphingomonas sequence or a high bacterial count does not by itself show disease suppression. A controlled comparison needs the particular bacterial strain, host and outcome. Differences in carbon-source use suggested competition as one possible mechanism, but did not exclude other explanations.[ref]
Protection of Arabidopsis thaliana against leaf-pathogenic Pseudomonas syringae by Sphingomonas strains in a controlled model system by Gerd Innerebner and others. Abstract, metabolic profiling and mechanistic qualification
Pseudomonas is a taxonomic genus of bacteria. Its type species is Pseudomonas aeruginosa, and it also contains Pseudomonas syringae. A type species anchors the genus name in biological nomenclature; it is not an assertion that every member has the same physiology or ecological role.[ref]
The distinction matters in plant experiments. A study of Pseudomonas syringae can identify the particular bacterial strain used, its host and its measured effects, without turning those results into a universal property of Pseudomonas. In a 2011 Arabidopsis thaliana study, researchers measured both growth of the challenge strain and disease symptoms. Those are biological outcomes attached to a specified experimental system.[ref]
For an identification example,
Pseudomonas sp. records genus-level assignment while leaving the species unspecified. Replacing that label with Pseudomonas aeruginosa adds information requiring evidence. Conversely, pooling counts from several Pseudomonas species answers a genus-level abundance question while concealing their individual contributions.Methylobacterium is a taxonomic genus of bacteria whose type species is Methylobacterium organophilum.[ref] The 1976 genus description concerned pink, rod-shaped isolates and emphasized facultative methylotrophy: using one-carbon compounds while also being able to grow with more complex organic carbon sources. That is a description of the original isolates, not a promise that every bacterial strain subsequently assigned to Methylobacterium has identical physiology.[ref]
Methylobacterium, a New Genus of Facultatively Methylotrophic Bacteria by T. E. Patt and others. Abstract, original isolates and carbon-source comparison
Plant experiments show why identifying the genus is only a starting point. In 2011, researchers established candidate bacterial colonists on Arabidopsis thaliana, then tested their effects during challenge by Pseudomonas syringae pv. tomato DC3000. The tested Methylobacterium strains did not reduce disease development, whereas some Sphingomonas isolates did.[ref]
Protection of Arabidopsis thaliana against leaf-pathogenic Pseudomonas syringae by Sphingomonas strains in a controlled model system by Gerd Innerebner and others. Abstract and Figure1, Methylobacterium comparison
The negative result distinguishes presence from protective function. Detecting a Methylobacterium sequence on a healthy leaf does not establish that the bacterium caused the leaf to remain healthy. Testing that idea needs a controlled comparison with a specified strain, host and outcome. Equally, failure to protect in this experiment does not rule out other interactions in different biological settings.
Mycobacterium smegmatis is a bacterial species used as an experimental model for mycobacterial molecular biology. Species identity and laboratory strain identity are different levels of description: the ATCC record for the genome-sequenced strain identifies a particular lineage, not every member of the species.[ref]
Mycobacterium smegmatis strain mc2 155 in the ATCC collection. ATCC700084, species, strain designation and genome-sequenced status
Its polynucleotide phosphorylase illustrates the model's scientific value. Researchers combined cryo-electron microscopy with enzyme assays to locate RNA in a three-subunit ring and test the contributions of selected amino acid residues. Comparing structure with catalytic measurements connects a molecular arrangement to a biochemical function.[ref]
Structure and mechanism of Mycobacterium smegmatis polynucleotide phosphorylase by Mihaela-Carmen Unciuleac and others. Abstract and Results, RNA-bound homotrimer and residue substitutions
For an original interpretation example, suppose a purified reference enzyme gives100 activity units and a substituted variant gives25 under the same stipulated assay conditions. The variant retains of that measured activity. This is evidence about the tested preparation and reaction; it does not by itself establish a fourfold change in bacterial growth or the same effect in Mycobacterium tuberculosis.
A node of Ranvier is a short gap between adjacent myelin sheaths along an axon. Its exposed cell membrane contains a high density of voltage-dependent sodium channels, allowing an action potential to be regenerated after local electric current has spread beneath the preceding sheath. Repetition of this arrangement supports saltatory conduction.[ref]
Anatomy and Physiology 2e by J. Gordon Betts and others. Sections 12.2, Myelin; 12.4, Propagation of the Action Potential
For a uniform bar in uniaxial linear elasticity, axial rigidity is the product of Young's modulus and load-bearing cross-sectional area. Since and ,Thus has units of force. It relates force to strain; axial stiffness relates force to extension over a specified length . The uniform-bar force-extension relation makes this distinction explicit.[ref]
Structural Stiffness Concept by TU Delft OpenCourseWare. Axial member relation delta=PL/(EA), structural stiffness and geometry
An original hollow-tube example keeps the area convention visible. Let the outer radius be , the inner radius and the stipulated modulus . The material area and rigidity areThe empty bore contributes no material area. An axial force gives strain . Tubes of lengths and have this same rigidity, but extensions and respectively. Their axial stiffnesses differ by a factor of two.
For a slowly varying area or modulus and constant axial force, extension instead accumulates locally:A single arithmetic average of generally cannot replace that compliance integral. The stepped-bar calculation in axial stiffness is its piecewise-constant counterpart.
A useful experiment measures axial force and strain between defined specimen points, over the proportional regime. The slope estimates rigidity under that loading model; converting a crosshead displacement directly into strain can mix in machine compliance or grip motion. The model also assumes that lateral constraints and end effects do not replace the intended uniaxial material response.
Markovization enlarges a state description until the conditional law of the next state depends only on the present enlarged state. For a second-order process with transition probabilities , define . The transition from goes to with probability .
A stationary sequence cycling through with uniformly random initial phase illustrates the lost information: after the current value zero, the next value depends on whether the preceding value was zero or one. The pairs follow the deterministic cycle . This finite-memory construction is useful, but a general process need not admit an economical finite-dimensional Markovization; retaining the entire history can be formally possible and computationally unhelpful.
Joseph A. Burns coauthored a study of radiation pressure and radiation-associated orbital drag on small Solar-System particles. Radiation pressure and radiation-associated orbital drag connect the microscopic exchange of photon momentum with long-term dust dynamics.[ref]
Radiation Forces on Small Particles in the Solar System by Joseph A. Burns and others. author list and the described study’s abstract or introductory account
Pinned article: Introduction to the OurBigBook Project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 2. You can publish local OurBigBook lightweight markup files to either OurBigBook.com or as a static website.Figure 3. Visual Studio Code extension installation.Figure 5. . You can also edit articles on the Web editor without installing anything locally. Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension. - Infinitely deep tables of contents:
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact





