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Density-gradient centrifugation uses a liquid whose density varies along the separation direction. A band does not by itself establish which mechanism produced it: rate-zonal centrifugation separates by migration rate before equilibrium, whereas isopycnic centrifugation uses equilibrium positions set by buoyant density.

For $F=(F_1,F_2,F_3)$ in Cartesian coordinates,

An incompressible flow preserves the volume of each moving fluid element. Its local condition is zero velocity divergence, $\nabla \cdot \mathbf{u}=0$. Combined with mass conservation, it says that each parcel's mass density stays unchanged as it moves. This does not require every parcel to have the same mass density: an initially nonuniform distribution can be transported without compression. Nor does incompressibility require a constant velocity or constant pressure.

For positive mass density, the continuity equation makes the equivalence precise:The material derivative follows a fluid element. Thus $D\rho /Dt=0$ if and only if the velocity field is a divergence-free vector field. The distinction between following a parcel and observing a fixed position prevents confusing incompressibility with an unchanging density field. ^[ref]

For a rigid pipe with impermeable walls, no leaks and steady flow, mass conservation at constant mass density implies a constant volumetric flow rate:Here $\mathbf{n}$ points downstream, $A$ is the section's area and $\overline{U}$ is the mean normal velocity, not necessarily the centreline speed. No assumption of inviscid flow or a uniform velocity profile is needed. If unequal amounts entered and left a fixed segment, its mass would accumulate, contradicting steady flow.

For a worked example, take horizontal water flow with stipulated mass density $1000\mathrm{kg}{\mathrm{m}}^{-3}$, inlet radius $R_1=0.010\mathrm{m}$, outlet radius $R_2=0.005\mathrm{m}$ and inlet mean velocity ${\overline{U}}_1=0.20\mathrm{m}{\mathrm{s}}^{-1}$. The circular areas giveThe radius halves, the area falls to one quarter, and the mean velocity quadruples. The mass flow rate is $\rho Q=0.02\pi \mathrm{kg}{\mathrm{s}}^{-1}$. The same amount of water passes both sections each second; narrowing changes its speed rather than compressing it. The Reynolds number and Bernoulli's principle discussions use this same example to examine viscous effects and the additional assumptions needed to calculate pressure.

An integral accumulates a function over a domain and may be defined as a limit of finite sums.

In isopycnic centrifugation, a species concentrates near the position where the liquid's density matches its buoyant density. In a density gradient increasing outward, material displaced inward encounters less-dense liquid and tends to move outward; material displaced outward encounters denser liquid and tends to move inward. Diffusion broadens the resulting band. If the density gradient never reaches the species' buoyant density, a matching equilibrium position need not exist within the vessel.^[ref]

The 1958 paper The replication of DNA in Escherichia coli by Matthew Meselson and Franklin W. Stahl (pp. 672–676, Figures 1–4) makes the distinction concrete. Its separation apparatus was a Spinco Model E ultracentrifuge with an Escherichia coli lysate in a cesium chloride density gradient. Ultraviolet wave absorption photographs of the rotating sample were scanned with a recording microdensitometer: peak position reported buoyant density, while signal above baseline reported DNA concentration.^[ref]

Mixed lysates labeled fully with the nitrogen isotope ${}^{15}\mathrm{N}$ and unlabeled lysates supplied reference bands; a mixture including intermediate material checked its position relative to those references. Figure 1 followed band formation over time, helping distinguish a migrating distribution from an approximately stationary one. These controls address resolution, calibration and approach to equilibrium. They do not make every ultraviolet-absorbing component identifiable as DNA, or turn a band into a direct image of individual strands. The DNA replication article develops the copying inference; here the key result is a separation by buoyant density, not a claim that heavier-labeled DNA is physically larger.^[ref]

The Oppenheimer-Snyder model describes a homogeneous ball of pressureless matter collapsing from rest, with a closed FLRW metric inside and a Schwarzschild metric outside. In geometric units its interior is $d{s}^2=-d{\tau }^2+a^2(d{\chi }^2+{\sin }^2\chi d{\Omega }^2)$, with $a=a_{\max }(1+\cos \eta )/2$ and $\tau =a_{\max }(\eta +\sin \eta )/2$. The boundary is a fixed comoving angle. Density conservation gives $\rho a^3$ constant, and the Friedmann equation at the initial turning point gives $8\pi {\rho }_0{a}_{\max }^2/3=1$.