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Joined 2026-09-21 Message
Incoming links: Complementary DNA
Complementary DNA 2026-10-14
Complementary DNA is DNA made from an RNA template by reverse transcription. When its template is mature messenger RNA, its sequence reflects the processed transcript rather than every intervening intron in the corresponding gene.
Reverse transcription 2026-10-14
Reverse transcription makes DNA from an RNA template using a reverse transcriptase. It reverses the template/product relationship of ordinary transcription, which makes RNA from DNA. The resulting Complementary DNA can supply a template for PCR amplification.[ref]
Basic Principles of RT-qPCR by Thermo Fisher Scientific. RNA as the Starting Material and Reverse Transcriptase Enzymes
RT-qPCR 2026-10-14
RT-qPCR applies qPCR after reverse transcription. Differences in RNA recovery and conversion into Complementary DNA can affect its inferred starting amount as well as differences in PCR efficiency.