Use the squared three-dimensional endpoint displacements, not their arithmetic mean. The mean squared displacement estimate and corresponding diffusion coefficient areThe factor six is for three-dimensional Brownian motion. For an isolated no-slip sphere, the Stokes–Einstein relation gives the Brownian radius estimate from endpoint displacements:Here room temperature is taken as ; choosing gives , a much smaller difference than the sampling uncertainty. Under independent increments with a normal distribution, for . The sampling uncertainty of an endpoint diffusivity estimate therefore makes this a rough radius estimate. It assumes negligible drift and hydrodynamic coupling between probes, the supplied bulk viscosity, and actual three-dimensional tracking. Localization noise adds to the squared displacements and would bias this inferred radius downward if uncorrected.
A synthetic fluid membrane is a thin, laterally fluid sheet whose components can rearrange in its plane while the sheet resists changes of area and curvature. Synthetic amphiphiles can assemble into two opposed layers with solvent-compatible groups facing outward. Their fluid in-plane response gives no equilibrium static shear modulus, but does not imply zero membrane bending rigidity or zero area-compression modulus. A thin membrane can therefore bend easily while remaining difficult to stretch. These notes cover generic synthetic interfaces.
A convenient continuum description is the Helfrich energy, augmented by spontaneous curvature and the Gaussian term:Here and are mean curvature and Gaussian curvature, is preferred curvature, and have energy units, and is membrane tension with units . Leaflet asymmetry can favor nonzero . At fixed topology the integrated Gaussian term is constant for a closed surface by the Gauss-Bonnet theorem. A symmetric tensionless spherical sheet has mean-curvature bending energy , independent of its radius in this ideal model. Edge energy can favor closure, while fixed area and enclosed volume constrain the resulting shapes.
For a nearly flat sheet, its small-slope elastic membrane energy becomesWith , the equipartition theorem gives the membrane fluctuation spectrum under tension:Bending suppresses short wavelengths, whereas positive tension suppresses the longest undulations. Their crossover is . Measuring the spectrum can separate these two contributions, provided finite image resolution, projected versus true area, and external confinement are accounted for. The drawn modes below are synthetic calculations, not an image of a sample.

