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A physicist researching laser physics.^[ref]

An engineer who developed laser instrumentation at Laboratoire Charles Fabry.^[ref]

A helium-neon laser is a gas laser using a mixture of helium and neon.

An electrical engineer.^[ref]

Source credit; professional specialization unestablished.

A physicist who researched laser cooling and optical trapping at Bell Laboratories, then worked as a principal scientist at Intel.^[ref]

A physicist.^[ref][ref]

In 1995, Conly L. Rieder, Richard W. Cole, Alexey Khodjakov and Greenfield Sluder tested whether an unattached kinetochore supplies the inhibitory signal delaying anaphase. Living PtK1 biological cells on glass coverslips were mounted in Rose chambers at $35$–$37{}^{\circ }\mathrm{C}$. An inverted microscope with a $60\times$ objective recorded differential interference contrast microscopy video. A pulsed $532\mathrm{nm}$ laser focused through that objective destroyed selected chromosome regions. The readout was chromosome movement and anaphase onset before and after surgery.^[ref]

Destroying the last unattached kinetochore permitted anaphase without that chromosome aligning. Cutting chromosome arms served as a damage control: progression still awaited attachment of the last naturally one-pole-attached chromosome. Correlative electron microscopy checked the absence of the targeted kinetochore. Together these results located an inhibitory activity in or near the unattached kinetochore, rather than establishing a requirement for alignment itself.^[ref]

This intervention localized control; it did not identify every signaling molecule or guarantee faithful inheritance after damage. The cultured PtK1 preparation differs from the hypothetical $2n=4$ cartoon. Neither the cartoon nor this experiment establishes identical control in every species.

A laser generates amplified light through stimulated emission. Its name abbreviates “light amplification by stimulated emission of radiation”. Lasers supply the intense beams used in optical tweezers; optical coherence also makes them useful for wave interference measurements. ^[ref].

In the usual material picture, a gain medium stores energy supplied by pumping, for example by incident light or an electric current. Stimulated emission transfers that stored energy to a selected optical normal mode, while competing photon absorption removes light. Producing net optical gain requires a suitable population distribution, such as population inversion in the simple model below.

A familiar laser oscillator uses mirrors to return light through the gain medium. This optical feedback favours resonant normal modes; a partly transmitting mirror lets useful power escape. Feedback and mode selection have different implementations, including ring arrangements: every laser need not resemble the same two-mirror device. The laser threshold example treats just that device. ^{[ref] [ref]}.

The useful combination is concentrated power and controlled optical normal modes. It gives optical tweezers a localized source of force and wave interference measurements a source with useful optical coherence. A laser can nevertheless emit several frequencies; “laser” alone does not guarantee a single frequency. ^[ref].

An original experimental landmark is T. H. Maiman's 1960 report of stimulated optical radiation from optically pumped ruby at about $694.3\mathrm{nm}$. His paper describes both the pumping path and the observed emission, connecting stored material energy with amplified light. ^[ref].

Laser cooling uses laser light to reduce the random velocities of chemical atoms, and therefore their translational temperature. The striking ingredient is selective photon absorption: an atom moving towards a beam sees a Doppler shift that can make it absorb more strongly. Each absorbed photon transfers momentum along the beam; repeated absorption and spontaneous emission can then remove kinetic energy. Doppler cooling makes this selection with the atom's motion, while sub-Doppler cooling also exploits its internal quantum states.

Cooling makes chemical atoms move slowly enough for longer interrogation in atomic clocks and atom interferometry, and easier loading into an optical trap. The same radiation pressure that pushes an atom can cool it: with suitably tuned opposing beams, whichever direction the atom moves, it receives the stronger push against that motion.

Laser detuning is the difference between a laser angular frequency and a specified atomic resonance angular frequency. Red detuning has $\delta <0$; blue detuning has $\delta >0$. The sign matters in laser cooling: a moving atom can see a red-detuned laser closer to resonance because of the Doppler effect.